Visualization of bacteria-induced autophagy by live-imaging.
Project/Area Number |
15K15130
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Bacteriology (including mycology)
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Research Institution | Kyoto University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
野澤 孝志 京都大学, 医学研究科, 助教 (10598858)
相川 知宏 京都大学, 医学研究科, 助教 (70725499)
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Project Period (FY) |
2015-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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Keywords | A群レンサ球菌 / オートファジー / イメージング / Rabタンパク質 / ゼノファジー / 膜形成 |
Outline of Final Research Achievements |
In this study, we selected a probe for visualization of infection step using group A Streptococcus as a model, and introduced a multidimensional fluorescence imaging system. We found that Rab 17, Rab 30 and Rab35 could colocalized to bacteria-induced autophagosome specifically. In particular, it was revealed that Rab 35 was involved in the formation of bacteria-induced autophagosomes by direct binding to NDP 52, which is an adapter protein. These results indicate that some specific Rab proteins are important for bacteria-induced autophagy.
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Report
(3 results)
Research Products
(37 results)
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[Journal Article] LILRA2 is an innate immune sensor for microbially cleaved immunoglobulins.2016
Author(s)
Hirayasu K, Saito F, Suenaga T, Shida K, Arase N, Oikawa K, Yamaoka T, Murota H, Chibana H, Nagai H, Nakamura Y, Katayama I, Colonna M, Arase H.
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Journal Title
Nature Microbiology
Volume: -
Issue: 6
Pages: 1-7
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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