| Project/Area Number |
15K15132
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bacteriology (including mycology)
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| Research Institution | Kyoto Prefectural University |
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Principal Investigator |
Osada-Oka Mayuko 京都府立大学, 生命環境科学研究科, 准教授 (40347498)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 結核 / マクロファージ / エキソソーム / バイオマーカー / 潜在性結核 / exosome |
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| Outline of Final Research Achievements |
The asymptomatic group, which is called latent infection of Mycobacterium tuberculosis (LITB), is recognized as vitally important in controlling TB, because a majority of cases develop from latent infections. Thus, it has been required new diagnostic method to exactly establish LTBI. In this study, we examined the biomarker-targeted protein into macrophage-derived exosomes. After infection with active or latent Mycobacterium bovis Bacillus Calmette-Guerin, the expressions of both inflammatory and uninflammatory factor were up-regulated in RAW264.7 macrophages. Therefore, the protein were increased in exosomes from BCG-infected macrophages (BCG-exo) compared with exosomes from uninfected macrophages (none-exo). Moreover, it was identified 9 proteins, which is in BCG-exo derived from BCG-infected macrophages.
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