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Molecular mechanism analysis of dormant cells causing refractory nature of bacterial infections

Research Project

Project/Area Number 15K15134
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Bacteriology (including mycology)
Research InstitutionWaseda University

Principal Investigator

TSUNEDA Satoshi  早稲田大学, 理工学術院, 教授 (30281645)

Co-Investigator(Renkei-kenkyūsha) OKUDA Shujiro  新潟大学, 大学院医歯学総合研究科, 准教授 (00512310)
Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords大腸菌 / 休止細菌 / マイクロアレイ / 感染症 / 抗生物質 / 乳酸デヒドロゲナーゼ / 微生物
Outline of Final Research Achievements

Non-dividing dormant bacteria that can survive in the presence of antibiotics by pausing their metabolic activity, cause the refractory nature of bacterial infections. Here we constructed the recombinant Escherichia coli strain generating a fluorescence resonance energy transfer (FRET) signal from the polymerization of FtsZ (called the Z-ring) during cell division. Then, dormant cells and dividing cells were successfully separated based on the FRET signal using a fluorescence activated cell sorter. The dormant cells showed significantly higher tolerance toward ofloxacin than dividing cells. Transcriptional analysis revealed that the dormant cells promote lactate dehydrogenase to adapt to anaerobic metabolism. In addition, single cell analysis by use of a microfluidic device supported expression of lactate dehydrogenase induces dormant cells.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (8 results)

All 2017 2016 2015

All Presentation (8 results) (of which Invited: 2 results)

  • [Presentation] 河合祐人,一色理乃,山本尚輝,凌一葦,奥田修二郎,松本慎也,常田聡2017

    • Author(s)
      ldhA遺伝子の確率的な発現による大腸菌のpersister形成
    • Organizer
      第90回日本細菌学会総会
    • Place of Presentation
      仙台
    • Year and Date
      2017-03-19
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 好気呼吸と発酵のバランスが崩れるとpersisterが形成される2017

    • Author(s)
      山本尚輝,一色理乃,河合祐人,田中大器,関口哲志,松本慎也,常田聡
    • Organizer
      第90回日本細菌学会総会
    • Place of Presentation
      仙台
    • Year and Date
      2017-03-19
    • Related Report
      2016 Annual Research Report
  • [Presentation] 確率的なldhA発現に伴うpersister形成のメカニズム解明2017

    • Author(s)
      山本尚輝,一色理乃,河合祐人,田中大器,関口哲志,松本慎也,常田聡
    • Organizer
      第5回日本生物工学会東日本支部コロキウム
    • Place of Presentation
      東京
    • Related Report
      2016 Annual Research Report
  • [Presentation] ldhAの確率的な発現によるpersister形成と制御2016

    • Author(s)
      山本尚輝,一色理乃,河合祐人,田中大器,関口哲志,松本慎也,常田聡
    • Organizer
      第31回日本微生物生態学会
    • Place of Presentation
      横須賀
    • Year and Date
      2016-10-22
    • Related Report
      2016 Annual Research Report
  • [Presentation] 大腸菌のpersister形成を誘導する多様な経路2016

    • Author(s)
      河合祐人,一色理乃,山本尚輝,松本慎也,奥田修二郎,常田聡
    • Organizer
      第31回日本微生物生態学会
    • Place of Presentation
      横須賀
    • Year and Date
      2016-10-22
    • Related Report
      2016 Annual Research Report
  • [Presentation] 嫌気代謝遺伝子の発現ノイズによるPersister形成の解析2016

    • Author(s)
      河合祐人,一色理乃,千原康太郎,山本尚輝,松本慎也,常田聡
    • Organizer
      第30回日本バイオフィルム学会学術集会
    • Place of Presentation
      東京
    • Year and Date
      2016-07-02
    • Related Report
      2016 Annual Research Report
  • [Presentation] 遺伝子発現解析とシングルセル観察から探るDormant Persistersの正体2016

    • Author(s)
      常田聡,河合祐人,宮川聡史,一色理乃,松本慎也
    • Organizer
      第89回日本細菌学会総会
    • Place of Presentation
      大阪国際交流センター(大阪市天王寺区)
    • Year and Date
      2016-03-23
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] 遺伝子発現から探る休止細菌の分子機構2015

    • Author(s)
      河合祐人,宮川聡史,一色理乃,奥田修二郎,松本慎也,常田聡
    • Organizer
      第29回日本バイオフィルム学会学術集会
    • Place of Presentation
      ホテル竹島(愛知県蒲郡市)
    • Year and Date
      2015-07-10
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2018-03-22  

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