Antiviral mechanism of new endoperoxide compounds showing antimalarial activity
Project/Area Number |
15K15142
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Virology
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Research Institution | Okayama University |
Principal Investigator |
Kato Nobuyuki 岡山大学, 医歯薬学総合研究科, 教授 (40150883)
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Project Period (FY) |
2015-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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Keywords | ウイルス / 感染症 / 微生物 / 抗ウイルス剤 / 薬理作用機序 / 遺伝子解析 / マイクロアレイ解析 |
Outline of Final Research Achievements |
We have carried out the study to clarify the anti-hepatitis C virus (HCV) mechanism of new anti-HCV compounds (N-89 and its derivative N-251). From N-89- or N-251-sensitive HCV-RNA-replicating cells (sensitive cells), we first established the cell lines possessing a N-89- or N-251-resistant phenotype (resistant cells). Using the sensitive and resistant cells, we analyzed the action mechanism of these compounds. The results appeared that both host and viral factors contribute to almost the same degree of the resistance to these compounds. Genetic analysis of HCVs derived from the resistant cells detected amino acids substitutions, which might be involved in the acquisition of resistance against these compounds. cDNA microarray analysis selected gene candidates, which might contribute to resistant acquisition. In addition, we demonstrated that these compounds showed antiviral effects against Japanese encephalitis virus, hepatitis B virus, and hepatitis E virus.
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Report
(3 results)
Research Products
(10 results)
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[Journal Article] Effects of resistance-associated NS5A mutations in hepatitis C virus on viral production and susceptibility to antiviral reagents.2016
Author(s)
Nitta S, Asahina Y, Matsuda M, Yamada N, Sugiyama R, Masaki T, Suzuki R, Kato N, Watanabe M, Wakita T, Kato T.
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Journal Title
Sci Rep
Volume: 6
Issue: 1
Pages: 34652-34652
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Control of HCV Replication with iMIRs, a Novel Anti-RNAi Agent2015
Author(s)
Saori Itami, Yutaka Eguchi, Takayuki Mizutani, Eriko Aoki, Tadaaki Ohgi, Masahiko Kuroda, Takahiro Ochiya, Nobuyuki Kato, Hiroshi I Suzuki, Norifumi Kawada and Yoshiki Murakami
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Journal Title
Molecular Therapy Nucleic Acids
Volume: 4
Pages: e219-e219
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Involvement of FKBP6 in hepatitis C virus replication.2015
Author(s)
Kasai H, Kawakami Y, Yokoe H, Yoshimura K, Matsuda M, Yasumoto J, Maekawa S, Yamashita A, Tanaka T, Ikeda M, Kato N, Okamoto T, Matsuura Y, Sakamoto N, Enomoto N, Takeda S, Fujii H, Tsubuki M, Kusunoki M, Moriishi K.
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Journal Title
Scientific reports
Volume: 5
Issue: 1
Pages: 16699-16699
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Presentation] Antimalarial preclinical drugs, N-89 and N-251, overcome various DAAs-resistant HCVs.2015
Author(s)
Youki Ueda, Hiromichi Dansako, Shinya Satoh, Hye-Sook Kim, Hiroyuki Doi, Yusuke Wataya, Masanori Ikeda, Nobuyuki Kato.
Organizer
22nd International Symposium on Hepatitis C Virus and Related Viruses
Place of Presentation
Strasbourg, France
Year and Date
2015-10-09
Related Report
Int'l Joint Research