The role of a transcription factor TFEB in the innate immune system

• Project/Area Number: 15K15148
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Immunology
• Research Institution: The University of Tokyo
• Principal Investigator: MIYAKE Kensuke 東京大学, 医科学研究所, 教授 (60229812)
• Project Period (FY): 2015-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2015: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
• Keywords: 自然免疫 / Toll様受容体

Outline of Final Research Achievements

The present study focused on the role of a transcription factor TFEB, a master regulator of lysosomes, in Toll-like receptor (TLR) responses. In preliminary studies, TFEB overexpression upregulated TLR responses. However, overexpression by another expression vector did not activate TLR responses. Further study was therefore difficult to pursue. Instead, we focused on the role of mTOR, a regulator of TFEB, in TLR responses, and found that mTORC2 play a role in TLR3 responses.

Research Products

• [Journal Article] Guanosine and its modified derivatives are endogenous ligands for TLR7 (2016)
  • Author(s): Shibata T, Ohto U, Nomura S, Kibata K, Motoi Y, Zhang Y, Murakami Y, Fukui R, Ishimoto T, Sano S, Ito T, Shimizu T, Miyake K.
  • Journal Title: Int Immunol Volume: - Issue: 5 Pages: 211-222
  • DOI: 10.1093/intimm/dxv062
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] mTOR activation influences TLR3 signaling (2015)
  • Author(s): SATO Ryota, SAITOH Shin-Ichiro, SHIBATA Takuma, FUKUI Ryutaro, MURAKAMI Yusuke, KATO Akihisa, ARII Jun, KAWAGUCHI Yasushi, MIYAKE Kensuke
  • Organizer: 日本免疫学会学術集会
  • Place of Presentation: 札幌コンベンションセンター(北海道 札幌市）
  • Year and Date: 2015-12-18