Identification of precursors of innate lymphoid cells
| Project/Area Number |
15K15149
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Immunology
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| Research Institution | Hokkaido University (2016-2017)
The University of Tokyo (2015) |
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Principal Investigator |
Sawa Shinichiro 北海道大学, 遺伝子病制御研究所, 准教授 (80611756)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | 自然リンパ球 / 分化 / 網羅的遺伝子発現解析 / リンパ節形成 / RANK / RORgt / LTi前駆細胞 / 発生 |
|---|
| Outline of Final Research Achievements |
Lymphoid Tissue inducer (LTi) cell is a member of group 3 innate lymphoid cell required for lymph node organogenesis. So far, the developmental pathway of LTi cell was not precisely investigated, Additionally, the microenvironment required for the maturation of LTi cell was not identified yet. In this research, using comprehensive transcriptome analysis, I tried to identify factors required for LTi cell maturation in vivo.
In the lymph node anlagen, mesenchymal cells express RANKL and LTi cells express RANK, a receptor for RANKL. In vitro, RANK signal upregulates maturation markers of LTi cells. Moreover, LTi cell specific RANK deficient mice lack all the lymph nodes. From these results, I concluded that RANKL expressed by mesenchymal cells in the lymph node anlagen is the critical factor for the terminal differentiation of LTi cells in vivo.
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Report
(4 results)
Research Products
(35 results)
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[Journal Article] Lymphatic Endothelial Cells Control Initiation of Lymph Node Organogenesis.2017
Author(s)
Onder L, Morbe U, Pikor N, Novkovic M, Cheng HW Hehlgans T, Pfeffer K, Becher B, Waisman A, Rulicke T, Gommerman J, Mueller CG, Sawa S. Scandella E, Ludewig B.
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Journal Title
Immunity
Volume: 47(1)
Issue: 1
Pages: 80-92
DOI
Related Report
Int'l Joint Research
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[Journal Article] SLAM-associated protein favors the development of iNKT2 over iNKT17 cells2016
Author(s)
Marie-Laure Michel, Christelle Lenoir, Berangere Massot, Severine Diem, Benoit Pasquier, Shinichiro Sawa, Rachel,Rignault-Bricard, Agnes Lehuen, Gerard Eberl, Andre Veillette, Maria Leite-de-Moraes and Sylvain Latour
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Journal Title
European Journal of Immunology
Volume: 46
Issue: 9
Pages: 2162-74
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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