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Identification of Genes Controlling Treg Development by Combining the CRISPR-Cas9 System and Bone Marrow Chimeric Mouse Model

Research Project

Project/Area Number 15K15154
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Immunology
Research InstitutionOsaka University

Principal Investigator

Huang Jun  大阪大学, 免疫学フロンティア研究センター, 特任研究員(常勤) (00751207)

Co-Investigator(Renkei-kenkyūsha) Ohkura Naganari  大阪大学, 医学系研究科, 特任教授 (20300949)
Tanaka Atsushi  大阪大学, 免疫学フロンティア研究センター, 特任助教 (00724105)
Research Collaborator Kitagawa Yohko  
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywords免疫寛容 / 自己免疫疾患 / エピジェネティクス / Regulatory T cells / epigenetics / immune tolerance / autoimmune diseases / Treg development / Epigenetic regulation / Etiology of autoimmunity / Self tolerance / Autoimmunity
Outline of Final Research Achievements

Regulatory T cells (Tregs) play an indispensable role in maintaining immune homeostasis. Understanding the molecular basis governing their development will help to understand the pathogenesis of autoimmune diseases and establish new strategies for the treatment and prevention of immunological diseases. We found that Mbd3 plays an essential role in thymic Treg development, and thus establishment of immune homeostasis. Mice with T cell-specific Mbd3 deletion have impaired Treg development, development multiple autoimmune diseases and die prematurely. Development of autoimmune diseases could be prevented by adoptive transfer of wild type Tregs. The requirement of Mbd3 for Tregs is restricted at developmental stage, since mice with Treg-specific deletion of Mbd3 could largely maintain their immune homeostasis. Our findings expand the notion that the defects in genetic and epigenetic control of thymic Tregs development could be potentially the cause of a wide range of autoimmune diseases.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (1 results)

All 2016

All Presentation (1 results) (of which Int'l Joint Research: 1 results)

  • [Presentation] MBD3, A COMPONENT OF THE NURD COMPLEX, SHAPES THE T-CELL RECEPTOR REPERTOIRE OF REGULATORY T CELLS2016

    • Author(s)
      Jun Huang, Yohko Kitagawa, Atsushi Tanaka, Naganari Ohkura,Shimon Sakaguchi
    • Organizer
      3rd Cold Spring Harbor Laboratory meeting on Epigenetics & Chromatin
    • Place of Presentation
      Cold Spring Harbor Laboratory, NY, USA
    • Year and Date
      2016-09-13
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research

URL: 

Published: 2015-04-16   Modified: 2019-12-27  

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