| Project/Area Number |
15K15193
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory medicine
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| Research Institution | Okayama University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
吉田 龍一 岡山大学, 大学病院, 助教 (80534768)
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| Co-Investigator(Renkei-kenkyūsha) |
KURODA Shinji 岡山大学, 大学病院, 助教 (60633758)
NAGASAKA Takeshi 岡山大学, 大学病院, 講師 (30452569)
TAZAWA Hiroshi 岡山大学, 大学病院, 准教授 (90415513)
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| Research Collaborator |
FUJIWARA Toshiyoshi 岡山大学, 医歯薬学総合研究科, 教授 (00304303)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 消化器癌 / 腹膜播種 / 癌微小環境 / マクロファージ / 抗癌剤抵抗性 / 腫瘍微小環境 / 膵癌 / 腹腔内遊離癌細胞 / 腫瘍関連マクロファージ |
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| Outline of Final Research Achievements |
Gastrointestinal cancer develops peritoneal dissemination, which is known to be refractory to current therapy. To overcome it, cancer microenvironment is need to be analyzed. For analysis of cells constituting the intraperitoneal microenvironment, we obtained peritoneal lavage fluid from patients with stomach cancer and pancreatic cancer and employed cancer-specific GFP expression virus TelomeScan to label cancer cell and immunostaining, which revealed the prevalence of tumor-associated macrophages (TAM) in the peritoneal cavity. Under the co-culture of stomach cancer or pancreatic cancer cells with TAM, the epithelial to mesenchymal transition of cancer cells, the enhancement of malignant phenotypes such as infiltration, migration, and acquired resistance to the chemotherapeutic agents were observed. These results suggested that intraperitoneal microenvironment, especially TAM, may contribute to the refractoriness of gastrointestinal tumor peritoneal dissemination.
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