Development of microdevice-based high throughput and high sensitivity in situ mRNA analysis method
| Project/Area Number |
15K15199
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Laboratory medicine
|
|---|
| Research Institution | Japan Women's University |
|---|
Principal Investigator |
Sato Kae 日本女子大学, 理学部, 准教授 (40373310)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
NISHIHARA Hiroshi 北海道大学, 医学研究科, 教授 (50322805)
|
|---|
| Project Period (FY) |
2015-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
|---|
| Keywords | 遺伝子分析 / マイクロデバイス / DNA / マイクロ流路 / mRNA / Padlock probe / 組織切片 |
|---|
| Outline of Final Research Achievements |
In this study, we aimed to improve the efficiency of padlock/RCA by determining the effects of microchannel shape and ultrasonic solution mixing. Using a circular-shaped microchamber and ultrasonic mixing, the efficiency of microfluidic padlock/RCA was improved, and the consumption of the expensive probe solution was reduced from 10 uL to approximately 3.5 uL. Moreover, the fluorescent probe hybridization time was reduced to 5 min, which is four times faster than that of the standard protocol. We used this method to successfully detect mitochondrial DNA and transcripts of β-actin and K-ras proto-oncogene codon 12 in cells.
|
Report
(4 results)
Research Products
(5 results)
