| Project/Area Number |
15K15200
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory medicine
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| Research Institution | St. Marianna University School of Medicine |
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Principal Investigator |
Sato Toshiyuki 聖マリアンナ医科大学, 医学部, 助教 (10350430)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 脱糖鎖 / N型糖鎖 / 自己抗体 / 関節リウマチ / 末梢血単核球 / 自己免疫疾患 / 蛋白質同定 / 糖鎖修飾 |
|---|
| Outline of Final Research Achievements |
In this study, we tried to identify aberrantly glycosylated proteins through detection of autoantibodies to the proteins. For this aim, we targeted the autoantibodies against proteins derived from peripheral blood mononuclear cells (PBMC) in rheumatoid arthritis (RA). We first tried to deglycosylate PBMC proteins from healthy donors, which were transferred to the membranes after SDS polyacrylamide gel electrophoresis. However, it was difficult to deglycosylate proteins on the membranes. Next, we tried to deglycosylate the PBMC proteins in vitro. The proteins were deglycosylated by peptide-N-glycosidase F. Sera from RA patients were applied to the deglycosylated PBMC proteins. However, the aimed autoantibodies were unfortunately not detected. In near future, serum proteins that contains more glycoproteins will be deglycosylated, and RA sera will be applied to the deglycosylated proteins. We will try to identify the RA-serum-reacted proteins as RA-specific aberrant glycosylated proteins.
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