| Project/Area Number |
15K15204
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pain science
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| Research Institution | Chubu University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
片野坂 友紀 岡山大学, 医歯薬学総合研究科, 助教 (60432639)
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| Research Collaborator |
HIBINO Yuhei 中部大学, 大学院・生命健康科学研究科, 大学院生
SHIMIZU Rie 中部大学, 生命健康科学部, 研究補助員
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 温度受容体 / 熱痛覚 / 一次感覚ニューロン / 侵害受容器 / TRPV1 / TRPV2 / CGRP / DRG / レセプター / 後根神経節 / 一次知覚神経 |
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| Outline of Final Research Achievements |
In this research, we utilized heat-dependent phosphorylation of ERK kinase in nociceptive primary sensory neurons, to explore an unknown heat-sensitive neuron that detects noxious heat higher than 50 degree C. In DRG neurons of TRPV1-KO mice, phosphorylated ERK (pERK) was emmerged after heat stimuli over 50 degree C, sugesting that these neurons would be the nociceptors specific to intensive heat. A substantial part of these pERK-positive neurons in TRPV1-KO mice were CGRP-positive peptidergic neurons, but IB4-negative. In addition, all of the cultured DRG neurons that exhibited heat threshold higher than 50 degree C were completely TRPV2-negative as far as we examined. As shown in these results, using pERK as an indicator of nociceptor activation, we succseded in identification of a certain type of nociceptor specialized to detect a particular stimulus. And thus, we have finally identified and characterized the inteisive heat-sensitive nociceptor, which is independent of TRPV1 and V2.
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