Establishment of the way to identify and isolate the heat-sensitive primary sensory neurons for understanding the peripheral mechanisms of heat pain.
| Project/Area Number |
15K15204
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pain science
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| Research Institution | Chubu University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
片野坂 友紀 岡山大学, 医歯薬学総合研究科, 助教 (60432639)
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| Research Collaborator |
HIBINO Yuhei 中部大学, 大学院・生命健康科学研究科, 大学院生
SHIMIZU Rie 中部大学, 生命健康科学部, 研究補助員
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 温度受容体 / 熱痛覚 / 一次感覚ニューロン / 侵害受容器 / TRPV1 / TRPV2 / CGRP / DRG / レセプター / 後根神経節 / 一次知覚神経 |
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| Outline of Final Research Achievements |
In this research, we utilized heat-dependent phosphorylation of ERK kinase in nociceptive primary sensory neurons, to explore an unknown heat-sensitive neuron that detects noxious heat higher than 50 degree C. In DRG neurons of TRPV1-KO mice, phosphorylated ERK (pERK) was emmerged after heat stimuli over 50 degree C, sugesting that these neurons would be the nociceptors specific to intensive heat. A substantial part of these pERK-positive neurons in TRPV1-KO mice were CGRP-positive peptidergic neurons, but IB4-negative. In addition, all of the cultured DRG neurons that exhibited heat threshold higher than 50 degree C were completely TRPV2-negative as far as we examined. As shown in these results, using pERK as an indicator of nociceptor activation, we succseded in identification of a certain type of nociceptor specialized to detect a particular stimulus. And thus, we have finally identified and characterized the inteisive heat-sensitive nociceptor, which is independent of TRPV1 and V2.
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Report
(3 results)
Research Products
(7 results)
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[Presentation] Peripheral pain mechanisms via neurotrophic factors in a rat reserpine-induced fibromyalgia model2017
Author(s)
Wakatsuki, K., Matsubara, T., Katanosaka, K., Yamanaka, A., Nishijo, H., Mizumura, K., Taguchi, T.
Organizer
第94回日本生理学会大会
Place of Presentation
アクトシティ―浜松（静岡県・浜松市）
Year and Date
2017-03-28
Related Report
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[Presentation] Nerve growth factor-mediated mechanism, but not muscle damage, is required for the induction of delayed onset muscle soreness in rats.2015
Author(s)
Hayashi, K., Katanosaka, K., Abe, M., Yamanaka, A., Mizumura, K. and Taguchi, T.
Organizer
第38回日本神経科学会
Place of Presentation
神戸国際会議場・国際展示場（兵庫県神戸市）
Year and Date
2015-07-29
Related Report
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