• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Extracellular miRNA causes neuropathic pain via spinal TLR7 in peripheral nerve injury.

Research Project

Project/Area Number 15K15206
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Pain science
Research InstitutionHiroshima Bunka Gakuen University

Principal Investigator

Morita Kastuya  広島文化学園大学, 看護学部, 教授 (10116684)

Co-Investigator(Kenkyū-buntansha) 土肥 敏博  広島文化学園大学, 看護学部, 教授 (00034182)
北山 友也  武庫川女子大学, 薬学部, 講師 (60363082)
本山 直世  広島大学, 医歯薬保健学研究院(歯), 助教 (70509661)
Research Collaborator SANAI MARI  広島文化学園大学, 看護学部, 教授 (20412330)
HASHIMOTO MIDORI  広島文化学園大学, 看護学部, 准教授 (60735257)
YAMADA AKIKO  広島文化学園大学, 看護学部, 講師 (20738174)
UEBAYASHI SATOKO  広島文化学園大学, 看護学部, 助教 (90633165)
OTSUBO KANAE  広島文化学園大学, 看護学部, 非常勤講師 (00461319)
TSUCHII AKIKO  広島文化学園大学, 看護学部, 学部学生
MINATO MAKI  広島文化学園大学, 看護学部, 学部学生
JODO YUI  広島文化学園大学, 看護学部, 学部学生
SHIGAKI MAYU  広島文化学園大学, 看護学部, 学部学生
SHIGEMOTO MIO  広島文化学園大学, 看護学部, 学部学生
KAKIMOTO YASUMASA  広島文化学園大学, 看護学部, 学部学生
Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
KeywordsマイクロRNA / Let-7b-5p / TLR7 / 鎮痛 / 神経障害性疼痛 / 難治性疼痛 / miRNA / 血小板活性化因子(PAF) / 誘導型PAF合成酵素(LPCAT2) / 疼痛緩和 / Let-7b / TLR-7 / LPCAT2
Outline of Final Research Achievements

Recent studies have shown that let-7b (miRNA lethal-7b) can be released from DRG neuron and intraplantar injection of let-7b-5p elicits rapid spontaneous pain via TLR7 and let-7b-5p can activates microglia and astrocytes via activation of TLR7. In the present study, we focused on the possible role of let-7b-5p, abundant in neuron, released into spinal cord by peripheral neuronal damage and contribute to the development of neuropathic pain via activation of spinal microglia and astrocytes. Intrathecal application of let-7b-5p and TLR7 agonists elicited sever and long lasting allodynia. The allodynia response to let-7b-5p and TLR7 agonists was abolished by intrathecal injection of siRNA of TLR7 mRNA. Allodynia response in neuropathic pain models by peripheral nerve injury was abolished by let-7b-5p against and the knockdown of spinal TLR7. The present results suggest that miRNA secreted in spinal cord by peripheral nerve injury participate in the development of neuropathic pain via TLR7.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (5 results)

All 2017 2016 2015

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (4 results)

  • [Journal Article] Down-regulation of zinc transporter-1 in astrocytes induces neuropathic pain via the brain-derived neurotrophic factor - K+-Cl- co-transporter-2 signaling pathway in the mouse spinal cord.2016

    • Author(s)
      Kitayama T, Morita K, Motoyama N, Dohi T.
    • Journal Title

      Neurochem Int.

      Volume: 101 Pages: 120-131

    • DOI

      10.1016/j.neuint.2016.11.001

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Extracellular miRNA causes neuropathic pain via spinal TLR7 in peripheral nerve injury.「神経障害性疼痛の発症における脊髄miRNA/TLR7系の役割」2017

    • Author(s)
      森田克也,本山直世,土肥敏博
    • Organizer
      第90回日本薬理学年会
    • Place of Presentation
      長崎
    • Year and Date
      2017-03-15
    • Related Report
      2016 Annual Research Report
  • [Presentation] 血小板活性化因子(PAF)合成酵素LPCAT2の難治性疼痛における役割2016

    • Author(s)
      本山直世,森田克也,土肥敏博
    • Organizer
      第36回 日本歯科薬物療法学会学術大会
    • Place of Presentation
      新潟
    • Year and Date
      2016-06-18
    • Related Report
      2016 Annual Research Report
  • [Presentation] Role of spinal LPCAT2, an inducible PAF synthesis enzyme on development and maintenance of painful peripheral neuropathy models in mice 「神経障害性疼痛の発症と維持機構における誘導型血小板活性化因子(PAF)合成酵素LPCAT2の役割」2016

    • Author(s)
      Morita K, Motoyama N, Kitayama T, Kanematsu T and Dohi T
    • Organizer
      第89回日本薬理学年会
    • Place of Presentation
      横浜
    • Year and Date
      2016-03-09
    • Related Report
      2015 Research-status Report
  • [Presentation] 難治性疼痛の発症と維持における血小板活性化因子(PAF)合成酵素LPCAT2の役割 「Role of the novel Platelet-Activating factor (PAF) synthetase LPCAT2 on chronic pain in mouse models」2015

    • Author(s)
      本山直世,森田克也,北山友也,柴 秀樹,土肥敏博
    • Organizer
      第142回日本歯科保存学会 2015年度春季学術大会
    • Place of Presentation
      北九州
    • Year and Date
      2015-06-25
    • Related Report
      2015 Research-status Report

URL: 

Published: 2015-04-16   Modified: 2020-03-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi