| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
To get useful information for development of norovirus vaccine, virus-like particles (VLPs) derived from several strains of norovirus were prepared and used to immunize rats. Rats sera reacted with VLPs used as antigens, but did not reacted with VLPs from other strains. It will be important to develop effective vaccines which can control wide variety of norovirus strains.
I tested whether norovirus VLPs could be used as exogenous antigen carriers. Although recombinant baculoviruses including the gene encoding a VP1 protein with a short peptide antigen inserted, no or extremely low expression of recombinant VLPs were found. It is suggested that the interaction between domains in the VP1 protein is important for the formation of a VLP.
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