| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
Apolipoprotein E-deficient (ApoE-/-) mice treated with double wall carbon nanotubes (DWCNTs) at the high dose showed increased plaque area in the aorta by oil red O staining. The expression of NLRP3, which is a central constitutive factor of inflammasome, and the expression of inflammatory factors (VCAM-1, ICAM-1) and cytokine (MCP-1) were significantly increased in ApoE-/- mice treated with long DWCNTs at the high dose. Furthermore, in human umbilical vein endothelial cells (HUVECs) treated with the high dose of DWCNTs the level of cytokine (IL-1β) was significantly elevated. The results suggested that DWCNTs induced the formation of arteriosclerotic lesion due to enhancing inflammatory responses triggered by inflammasome activation.
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