Human induced pluripotent stem cell-derived hepatic cell lines as a new model for host interaction with hepatitis B virus

• Project/Area Number: 15K15285
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Gastroenterology
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: Asahina Yasuhiro 東京医科歯科大学, 大学院医歯学総合研究科, 寄附講座教授 (00422692)
• Co-Investigator(Kenkyū-buntansha): 柿沼 晴 東京医科歯科大学, 大学院医歯学総合研究科, 寄附講座講師 (30372444)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: HBV / Human iPS cell / cccDNA / iPS細胞 / B型肝炎ウイルス / NTCP / 肝幹・前駆細胞 / 幹細胞分化

Outline of Final Research Achievements

HBV is not eradicated by current antiviral therapies due to persistence of HBV cccDNA in host cells, and thus development of novel culture models for productive HBV infection is urgently needed, which will allow the study of HBV cccDNA eradication. To meet this need, we developed culture models of HBV infection using human induced pluripotent stem cell-derived hepatocyte lineages, including immature proliferating hepatic progenitor-like cell lines and differentiated hepatocyte-like cells. These cells were susceptible to HBV infection, produced HBV particles, and maintained innate immune responses. This study provides evidence that iPS-derived hepatic cell lines can be utilized for novel HBV culture models with genetic variation to investigate the interactions between HBV and host cells and the development of anti-HBV strategies.

Research Products

• [Journal Article] Hepatitis B Surface Antigen Reduction by Switching from Long-term Nucleoside/nucleotide Analog Administration to Pegylated Interferon. (2017)
  • Author(s): Tamaki N, Kurosaki M, Kusakabe A, Orito E, Joko K, Kojima Y, Kimura H, Uchida Y, Hasebe C, Asahina Y, Izumi N.
  • Journal Title: J Viral Hepat Volume: 印刷中 Issue: 8 Pages: 672-678
  • DOI: 10.1111/jvh.12691
  • Peer Reviewed
• [Journal Article] Genome-wide Association Study Identifies TLL1 Variant Associated With Development of Hepatocellular Carcinoma After Eradication of Hepatitis C Virus Infection. (2017)
  • Author(s): Matsuura K, Honda M, et al.
  • Journal Title: Gastroenterology. Volume: - Issue: 6 Pages: 1383-1394
  • DOI: 10.1053/j.gastro.2017.01.041
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] ITPA gene variation and ribavirin-induced anemia in patients with genotype 2 chronic hepatitis C treated with sofosbuvir plus ribavirin. (2017)
  • Author(s): Murakawa M, Asahina Y, Nagata H, Nakagawa M, Kakinuma S, Nitta S, Kawai-Kitahata F, Otani S, Kaneko S, Miyoshi M, Tsunoda T, Asano Y, Sato A, Itsui Y, Azuma S, Nouchi T, Furumoto Y, Asano T, Chuganji Y, Tohda S, Watanabe M.
  • Journal Title: Hepatol Res Volume: 印刷中 Issue: 11 Pages: 1212-1218
  • DOI: 10.1111/hepr.12867
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Hepatic IFNL4 expression is associated with non-response to interferonbased therapy through the regulation of basal interferon-stimulated gene expression in chronic hepatitis C patients. (2017)
  • Author(s): Murakawa M, Asahina Y, Kawai-Kitahata F, Nakagawa M, Nitta S, Otani S, Nagata H, Kaneko S, Asano Y, Tsunoda T, Miyoshi M, Itsui Y, Azuma S, Kakinuma S, Tanaka Y, Iijima S, Tsuchiya K, Izumi N, Tohda S, Watanabe M.
  • Journal Title: J Med Virol Volume: in press Issue: 7 Pages: 1241-1247
  • DOI: 10.1002/jmv.24763
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Complex pattern of resistance-associated substitutions of hepatitis C virus after daclatasvir/asunaprevir treatment failure. (2017)
  • Author(s): Itakura J, Kurosaki M, Hasebe C, Osaki Y, Joko K, Yagisawa H, Sakita S, Okushin H, Satou T, Hisai H, Abe T, Tsuji K, Tamada T, Kobashi H, Mitsuda A, Ide Y, Ogawa C, Tsuruta S, Takaguchi K, Murakawa M, Asahina Y, Enomoto N, Izumi N.
  • Journal Title: PLoS One Volume: － Issue: 10 Pages: e0165339-e0165339
  • DOI: 10.1371/journal.pone.0165339
  • Peer Reviewed / Open Access
• [Journal Article] Effects of resistance-associated NS5A mutations in hepatitis C virus on viral production and susceptibility to antiviral reagents. (2016)
  • Author(s): Nitta S, Asahina Y, Matsuda M, Yamada N, Sugiyama R, Masaki T, Suzuki R, Kato N, Watanabe M, Wakita T, Kato T.
  • Journal Title: Sci Rep Volume: 6 Issue: 1 Pages: 34652-34652
  • DOI: 10.1038/srep34652
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Bone Morphogenetic Protein-4 Modulates Proliferation and Terminal Differentiation of Fetal Hepatic Stem/Progenitor Cells. (2016)
  • Author(s): Goto F, Kakinuma S, Miyoshi M, Tsunoda T, Kaneko S, Sato A, Asano Y, Otani S, Azuma S, Nagata H, Kawai-Kitahata F, Murakawa M, Nitta S, Itsui Y, Nakagawa M, Asahina Y, Watanabe M.
  • Journal Title: Hepatol Res Volume: 印刷中 Issue: 9 Pages: 941-952
  • DOI: 10.1111/hepr.12823
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Irregular vascular pattern by contrast-enhanced ultrasonography and high serum Lens culinaris agglutinin-reactive fraction of alpha-fetoprotein level predict poor outcome after successful radiofrequency ablation in patients with early-stage hepatocellular carcinoma. (2016)
  • Author(s): Takada H, Tsuchiya K, Yasui Y, Nakakuki N, Tamaki N, Nakanishi H, Itakura J, Takahashi Y, Kurosaki M, Asahina Y, Enomoto N, Izumi N.
  • Journal Title: Cancer Med Volume: 5 Issue: 11 Pages: 3111-3120
  • DOI: 10.1002/cam4.932
  • Peer Reviewed
• [Journal Article] Serial measurement of Wisteria floribunda agglutinin positive Mac-2-binding protein is useful for predicting liver fibrosis and the development of hepatocellular carcinoma in chronic hepatitis C patients treated with IFN-based and IFN-free therapy. (2016)
  • Author(s): Nagata H, Nakagawa M, Nishimura-Sakurai Y, Asano Y, Tsunoda T, Miyoshi M, Kaneko S, Goto F, Otani S, Kawai-Kitahata F, Murakawa M, Nitta S, Itsui Y, Seishin Azuma S, Kakinuma S, Tojo N, Tohda T, Asahina Y, Watanabe M, the Ochanomizu Liver Conference Study Group.
  • Journal Title: Hepatol Int Volume: 10 Issue: 6 Pages: 956-964
  • DOI: 10.1007/s12072-016-9754-1
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Human induced pluripotent stem cell-derived hepatic cell line as a new model for host interaction with hepatitis B virus. (2016)
  • Author(s): Kaneko S, Kakinuma S, Asahina Y, Kamiya A, Miyoshi M, Tsunoda T, Nitta S, Asano Y, Nagata H, Otani S, Kitahata F, Murakawa M, Itsui Y, Nakagawa M, Azuma S, Nakauchi H, Iwamoto M, Watashi K, Wakita T, Sugiyama M, Mizokami M, Shimotohno K, Watanabe M.
  • Journal Title: Sci Rep Volume: ６ Issue: 1 Pages: 29358-29358
  • DOI: 10.1038/srep29358
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Evaluation of IFN resistance in newly established genotype 1b HCV cell culture system. (2016)
  • Author(s): Taniguchi M, Tasaka-Fujita M, Nakagawa M, Watanabe T, Kawai-Kitahata F, Otani S, Goto F, Nagata H, Kaneko S, Nitta S, Murakawa M, Nishimura-Sakurai Y, Azuma S, Itsui Y, Mori K, Yagi S, Kakinuma S, Asahina Y, Watanabe M.
  • Journal Title: J Clin Transl Hepatol Volume: 4 Issue: 1 Pages: 5-11
  • DOI: 10.14218/jcth.2015.00047
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Matrix Metalloproteinase-14 Mediates Formation of Bile Ducts and Hepatic Maturation of Fetal Hepatic Progenitor Cells. (2016)
  • Author(s): Otani S, Kakinuma S, Kamiya A, Goto F, Kaneko S, Miyoshi M, Tsunoda T, Asano Y, Kawai-Kitahata F, Nitta S, Nakata T, Okamoto R, Itsui Y, Nakagawa M, Azuma S, Asahina Y, Yamaguchi T, Koshikawa N, Seiki M, Nakauchi H, Watanabe M.
  • Journal Title: Biochem Biophys Res Commun Volume: 469 Issue: 4 Pages: 1062-1068
  • DOI: 10.1016/j.bbrc.2015.12.105
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Comprehensive analyses of mutations and hepatitis B virus integration in hepatocellular carcinoma with clinicopathological features. (2016)
  • Author(s): Kawai-Kitahata F, Asahina Y, Tanaka S, Kakinuma S, Murakawa M, Sayuri Nitta S, Watanabe T, Otani S, Taniguchi M, Goto F, Nagata H, Kaneko S, Tasaka-Fujita M, Nishimura-Sakurai Y, Azuma S, Itsui Y, Nakagawa M, Tanabe M, Takano S, Fukasawa M, Sakamoto M, Maekawa S, Enomoto N, Watanabe M.
  • Journal Title: Journal of Gastroenterology Volume: 51 Issue: 5 Pages: 473-486
  • DOI: 10.1007/s00535-015-1126-4
  • Peer Reviewed
• [Journal Article] Efficacy of additional radiofrequency ablation after transcatheter arterial chemoembolization for intermediate hepatocellular carcinoma. (2016)
  • Author(s): Azuma S, Asahina Y, Nishimura-Sakurai Y, Kakinuma S, Kaneko S, Nagata H, Goto F, Ootani S, Kawai-Kitahata F, Taniguchi M, Murakawa M, Watanabe T, Tasaka-Fujita M, Itsui Y, Nakagawa M, Watanabe M.
  • Journal Title: Heptol Res Volume: 46 Issue: 4 Pages: 312-9
  • DOI: 10.1111/hepr.12566
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] JSH Guidelines for the Management of Hepatitis C Virus Infection: A 2016 update for genotype 1 and 2 (2016)
  • Author(s): Asahina Y, Izumi N, Hiromitsu K, Kurosaki M, Koike K, Suzuki F, Takikawa H, Tanaka A, Tanaka E, Tanaka Y, Tsubouchi H, Hayashi N, Hiramatsu N, Yotsuyanagi H
  • Journal Title: Hepatol Res Volume: 46 Issue: 2 Pages: 129
  • DOI: 10.1111/hepr.12645
  • Peer Reviewed
• [Journal Article] Need for appropriate programs for prevention of hepatitis B infection: lessons from the latest nationwide survey in Japan. (2016)
  • Author(s): Asahina Y, Watanabe M
  • Journal Title: J Gastroenterol Hepatol Volume: 31 Issue: 1 Pages: 12-13
  • DOI: 10.1111/jgh.13157
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Genetic polymorphisms of IL28B and PNPLA3 are predictive for HCV related rapid fibrosis progression and identify patients who require urgent antiviral treatment with new regimens. (2015)
  • Author(s): Tamaki N, Kurosaki M, Higuchi M, Takada T, Nakakuki N, Yasui Y, Suzuki S, Tsuchiya K, Nakanishi H, Itakura J, Takahashi Y, Ogawa S, Tanaka Y, Asahina Y, Izumi N
  • Journal Title: PLoS One Volume: 10 Issue: 9 Pages: e0137351-e0137351
  • DOI: 10.1371/journal.pone.0137351
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Amino acid polymorphisms in hepatitis C virus core affect infectious virus production and major histocompatibility complex class I molecule expression (2015)
  • Author(s): Tasaka-Fujita M, Sugiyama N, Kang W, Masaki T, Murayama A, Yamada N, Sugiyama R, Tsukuda S, Watashi K, Asahina Y, Sakamoto N, Wakita T, Shin EC, Kato T
  • Journal Title: Sci Rep Volume: 5 Issue: 1 Pages: 13994-14002
  • DOI: 10.1038/srep13994
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] Reduced changes in bone mineral density in CHB patients receiving tenofovir alfenamide (TAF) compared with tenofovir disoproxil fumarate. (2016)
  • Author(s): Seto WK, Asahina Y, Peng, Stanciu CYC, Abdurakhmanov D, Flaherty JF, Kim K, Gaggar A, Subramanian M, Tabak F, Tuan T. Nguyen TT, Chan HLT
  • Organizer: The 67th Annual Meeting of the American Association for the Study of Liver Disease (AASLD The Liver Meeting 2016).
  • Place of Presentation: Boston, USA
  • Year and Date: 2016-11-11
  • Int'l Joint Research / Invited
• [Presentation] Genetically modified human induced pluripotent stem cell-derived hepatic progenitor-like cell lines as a model for interaction between hepatitis В virus and host cells. (2016)
  • Author(s): Kaneko S, Kakinuma S, Asahina Y, Kamiya A, Miyoshi M, Tsunoda T, Nitta S, Sato A, Asano Y, Nagata H, Otani S, Kawai-Kitahata F, Murakawa M, Nakagawa M, Azuma S, Watanabe M.
  • Organizer: The 67th Annual Meeting of the American Association for the Study of Liver Disease (AASLD The Liver Meeting 2016).
  • Place of Presentation: Boston, USA
  • Year and Date: 2016-11-11
  • Invited
• [Presentation] B型肝炎治療のアップデート HBV排除にむけたヒトiPS細胞由来肝細胞系譜細胞を用いた培養系の開発． (2016)
  • Author(s): 金子 俊, 柿沼 晴, 朝比奈 靖浩
  • Organizer: 第20回日本肝臓学会大会
  • Place of Presentation: 神戸コンベンションセンター（兵庫県神戸市）
  • Year and Date: 2016-11-03
• [Presentation] Recent progress in anti-HCV therapy and challenges toward HCC eradication. (2016)
  • Author(s): Asahina Y.
  • Organizer: Academic Forum of Liver Disease in Northeast China and the Annual Convention for the Study of Liver Diseases in Jilin Province in 2016
  • Place of Presentation: Changchun, China
  • Year and Date: 2016-10-29
  • Int'l Joint Research / Invited
• [Presentation] Hepatocarcinogenesis after hepatitis C viral eradication. (2016)
  • Author(s): Asahina Y
  • Organizer: The 10th Asian Pacific Association for the Study of the Liver (APASL) Single Topic Conference
  • Place of Presentation: Kaoshiung（Taiwan）
  • Year and Date: 2016-06-08
  • Int'l Joint Research / Invited
• [Presentation] B型肝炎研究のUp-To-Date ヒトiPS細胞由来肝細胞系譜細胞を用いた新たなHBV感染培養系モデル (2016)
  • Author(s): 金子 俊, 柿沼 晴, 朝比奈 靖浩
  • Organizer: 第52回日本肝臓学会総会
  • Place of Presentation: ホテルニューオータニ幕張、東京ベイ幕張ホール（千葉県美浜区）
  • Year and Date: 2016-05-19
• [Presentation] B型肝炎ウイルス排除にむけて 創薬への新知見 ヒトiPS細胞由来肝細胞系譜細胞を用いた創薬を目指したHBV感染培養研究 (2016)
  • Author(s): 金子 俊, 朝比奈 靖浩, 渡辺 守
  • Organizer: 第102回日本消化器病学会総会
  • Place of Presentation: 京王プラザホテル（東京都新宿区）
  • Year and Date: 2016-04-21
• [Presentation] A model for interaction between HBV and host cells derived from human induced pluripotent stem cells. (2016)
  • Author(s): Kaneko S, Kakinuma S, Asahina Y, Kamiya A, Nitta S, Tsunoda T, Miyoshi M, Asano Y, Nagata H, Goto F, Otani S, Kitahata F, Murakawa M, Itsui Y, Nakagawa M, Azuma AS, Watanabe M.
  • Organizer: The 51th annual meeting of the European association for the study of the liver (EASL The International Liver Congress 2016)
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2016-04-13
  • Invited
• [Presentation] A model for interaction between HBV and host cells derived from human induced pluripotent stem cells. (2016)
  • Author(s): Kaneko S, Kakinuma S, Asahina Y, Kamiya A, Nitta S, Tsunoda T, Miyoshi M, Asano Y, Nagata H, Goto F, Otani S, Kitahata F, Murakawa M, Itsui Y, Nakagawa M, Azuma S, Watanabe M
  • Organizer: The 51th annual meeting of the European association for the study of the liver (EASL The International Liver Congress 2016)
  • Place of Presentation: Barcelona（Spain）
  • Year and Date: 2016-04-13
  • Int'l Joint Research
• [Presentation] Comprehensive analyses of mutations and hepatitis B virus integration in hepatocellular carcinoma. (2016)
  • Author(s): Kawai-Kitahata F, Asahina Y, Tanaka S, Kakinuma S, Murakawa M, Nitta S, Otani S, Goto F, Nagata H, Kaneko S, Asano Y, Miyoshi M, Tsunoda T, Azuma S, Itsui Y, Nakagawa M, Tanabe M, Maekawa S, Enomoto N, Watanabe M
  • Organizer: 25th conference of the Asian Pacific Association for the Study of the Liver (APASL 2016)
  • Place of Presentation: グランドプリンスホテル新高輪（東京都港区）
  • Year and Date: 2016-02-20
  • Int'l Joint Research
• [Presentation] A model for interaction between HBV and host cells derived from human induced pluripotent stem cells. (2016)
  • Author(s): Kaneko S, Kakinuma S, Asahina Y, Kamiya A, Nitta S, Tsunoda T, Miyoshi M, Asano Y, Nagata H, Goto F, Otani S, Kitahata F, Murakawa M, Itsui Y, Nakagawa M, Azuma S, Watanabe M.
  • Organizer: 25th conference of the Asian Pacific Association for the Study of the Liver (APASL 2016)
  • Place of Presentation: グランドプリンスホテル新高輪（東京都港区）
  • Year and Date: 2016-02-20
  • Int'l Joint Research
• [Presentation] Serial changes in M2BPGi levels as predictors of fibrosis and HCC in chronic hepatitis C. (2016)
  • Author(s): Nagata H, Sakurai Y, Nakagawa M, Kakinuma S, Azuma S, Murakawa M, Nitta S, Itsui Y, Asahina Y, Watanabe M
  • Organizer: 25th conference of the Asian Pacific Association for the Study of the Liver (APASL 2016)
  • Place of Presentation: グランドプリンスホテル新高輪（東京都港区）
  • Year and Date: 2016-02-20
  • Int'l Joint Research
• [Presentation] In vitro characterization and drug sensitivity analysis for HCV NS5A resistance-associated variants. (2016)
  • Author(s): Nitta S, Asahina Y, Wakita T, Kato T
  • Organizer: 25th conference of the Asian Pacific Association for the Study of the Liver (APASL 2016)
  • Place of Presentation: グランドプリンスホテル新高輪（東京都港区）
  • Year and Date: 2016-02-20
  • Int'l Joint Research
• [Presentation] Hepatocarcinogenesis after hepatitis C viral eradication (2016)
  • Author(s): Asahina Y
  • Organizer: The 10th APASL Single Topic Conference
  • Place of Presentation: Kaoshiung, Taiwan
  • Int'l Joint Research / Invited
• [Presentation] Human induce pluripotent stem cell-dereived hepatic progenitor-like cells and hepatocyte-like cells as a model for interaction between hepatitis B virus and host cells. (2015)
  • Author(s): Kaneko S, Kakinuma S, Asahina Y, Kamiya A, Nitta S, Tsunoda T, Miyosi M, Nagata H, Goto F, Otani S, Murakawa M, Kawai-Kitahata F, Itsui Y, Nakagawa M, Azuma S, Watanabe M
  • Organizer: The 66th Annual Meeting of the American Association for the Study of Liver Disease (AASLD The Liver Meeting 2015)
  • Place of Presentation: San Francisco（USA）
  • Year and Date: 2015-11-13
  • Int'l Joint Research
• [Presentation] Comprehensive analyses of mutations and hepatitis B virus integration in hepatocellular carcinoma with clinicopathological features. (2015)
  • Author(s): Kawai-Kitahata F, Asahina Y, Tanaka S, Kakinuma S, Murakawa M, Nitta S, Watanabe T, Otani S, Goto F, Nagata H, Kaneko S, Azuma S, Itsui Y, Nakagawa M, Tanabe M, Maekawa S, Enomoto N, Watanabe M
  • Organizer: The 66th Annual Meeting of the American Association for the Study of Liver Disease (AASLD The Liver Meeting 2015)
  • Place of Presentation: San Francisco（USA）
  • Year and Date: 2015-11-13
  • Int'l Joint Research
• [Presentation] Effects of resistance mutations of NS5A inhibitor on viral production and susceptibility to anti-HCV reagents in recombinant hepatitis C viruses with NS5A of genotype 1b. (2015)
  • Author(s): Nitta S, Asahina Y, Wakita T, Kato T
  • Organizer: The 66th Annual Meeting of the American Association for the Study of Liver Disease (AASLD The Liver Meeting 2015)
  • Place of Presentation: San Francisco（USA）
  • Year and Date: 2015-11-13
  • Int'l Joint Research
• [Presentation] Expression of IFN lambda 4 in liver is closely associated with non-response to antiviral therapy through the regulation of basal expression of ISGs in chronic hepatitis C patients but not in hepatitis B. (2015)
  • Author(s): Murakawa M, Asahina Y, Kawai-Kitahata F, Nagata H, Kaneko S, Nitta S, Watanabe T, Itsui Y, Nakagawa M, Kakinuma S, Iijima S, Tanaka Y, Watanabe M, Tanaka Y
  • Organizer: The 66th Annual Meeting of the American Association for the Study of Liver Disease (AASLD The Liver Meeting 2015)
  • Place of Presentation: San Francisco（USA）
  • Year and Date: 2015-11-13
  • Int'l Joint Research
• [Presentation] Serial change of resistant associated variants during early phase of NS3/4A triple therapy and the final virological outcome: analyses by ultra-deep sequenceing technology. (2015)
  • Author(s): Watanabe T, Asahina Y, Nakagawa M, Kakinuma S, Itsui Y, Nagata H, Murakawa M, Kawai-Kitahata F, Miura M, Maekawa S, Enomoto N, Watanabe M
  • Organizer: The 66th Annual Meeting of the American Association for the Study of Liver Disease (AASLD The Liver Meeting 2015)
  • Place of Presentation: San Francisco（USA）
  • Year and Date: 2015-11-13
  • Int'l Joint Research
• [Presentation] Variations of the genome and interaction of hepatitis B viral X protein associated with hepatocarcinogenesis. (2015)
  • Author(s): Nagata H, Itsui Y, Kawai-Kitahata F, Kaneko S, Murakawa M, Nitta S, Nakagawa M, Azuma S, Kakinuma S, Asahina Y
  • Organizer: The 66th Annual Meeting of the American Association for the Study of Liver Disease (AASLD The Liver Meeting 2015)
  • Place of Presentation: San Francisco（USA）
  • Year and Date: 2015-11-13
  • Int'l Joint Research