| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
In this study, we investigated the method to induce secretory-lineage differentiation of cultured human intestinal organoids, and also performed some functional analyses of those organoids. Consequently, we successfully established over 40-lines of human small intestinal- or colonic-organoids from endoscopic biopsy specimens. Short-term exposure to a gamma-secretase inhibitor, combined with depletion of Wnt3a, SB20190 and Nicotinamide, certainly directed human intestinal organoids towards secretory cell-lineage differentiation. We also established a quantitative analysis system to evaluate the response of human intestinal organoids to a secretagogue, PGE2, or an enterohormone, VIP, and showed their properties to regulate the body-fluid balance.
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