Single cell imaging in human intestinal stem cell
Project/Area Number |
15K15287
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Gastroenterology
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Research Institution | Tokyo Medical and Dental University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
渡辺 守 (渡邉 守) 東京医科歯科大学, 大学院医歯学総合研究科, 教授 (10175127)
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Project Period (FY) |
2015-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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Keywords | 1幹細胞可視化 / Lgr5プロモーター / 1幹細胞動態 |
Outline of Final Research Achievements |
We did random labeling of fluorescence into the organoid cells by original method. We then found single fluorescence-labeled cell in crypt and isolated it. The organoids could be generated from single fluorescence cell, indicating that single fluorescence cell might be intestinal stem cell. We could therefore observe the cell division of single fluorescence stem cell and lineage tracing of differentiated cell in three dimensions. Moreover, we evaluated the role between stem cells for the formation of one villus by analyzing the mutual relations with the fluorescence-negative stem cell and fluorescence stem cell. In addition, we started the evaluation of single stem cell using human intestinal organoids to estimate the regulation of single stem cell derived from the patients.
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Report
(3 results)
Research Products
(9 results)
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[Journal Article] Long-term inflammation transforms intestinal epithelial cells of colonic organoids.2017
Author(s)
Hibiya S, Tsuchiya K, Hayashi R, Horita N, Watanabe S, Shirasaki T, Nishimura R, Kimura N, Nishimura T, Gotoh N, Oshima S, Okamoto R, Nakamura T, Watanabe M
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Journal Title
J Crohns Colitis.
Volume: 61
Pages: 621-630
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Presentation] 消化管幹細胞研究の最前線2017
Author(s)
土屋輝一郎
Organizer
第13回日本消化管学会総会
Place of Presentation
名古屋国際会議場(愛知県名古屋市)
Year and Date
2017-03-17
Related Report
Invited
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