Metagenome analysis of intestinal virome in inflammatory bowel disease patients

• Project/Area Number: 15K15289
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Gastroenterology
• Research Institution: Kanazawa University
• Principal Investigator: IIDA Noriho 金沢大学, 医学系, 助教 (40705604)
• Co-Investigator(Kenkyū-buntansha): 北村 和哉 金沢大学, 附属病院, 助教 (00579633)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 炎症性腸疾患 / 炎症背腸疾患 / 潰瘍性大腸炎 / 腸内微生物叢 / メタゲノム / ウイルス

Outline of Final Research Achievements

Inflammatory bowel diseases including Crohn disease (CD) and ulcerative colitis (UC) reveal dysbiosis of gut flora and dysregulation of inflammation in the colon. Gut microbiota of CD or UC patients contained significantly differential bacterial species and virus compared with healthy donors according to metagenome analysis. Il10-deficient mice transplanted with feces of UC, CD patients showed more severe inflammation in the colon and higher expression of inflammatory cytokine including tnf, il6 and il1b than the mice transplanted with feces of healthy donors. Taken together, it is suggested that the dysbiotic gut microbiota of inflammatory bowel disease patients exacerbates colitis.

Research Products

• [Presentation] Identification of colitis-associated bacteria in intestine of inflammatory bowel disease patients (2017)
  • Author(s): 清島淳、飯田宗穂、金子周一
  • Organizer: The 13th Congress of European Crohn's and Colitis Organization
  • Int'l Joint Research