Development of an On-chip Simulator of Hepatic Lobule
| Project/Area Number |
15K15299
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Gastroenterology
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| Research Institution | Tokai University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
紙谷 聡英 東海大学, 医学部, 准教授 (30321904)
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| Co-Investigator(Renkei-kenkyūsha) |
KIMURA Hiroshi 東海大学, 工学部, 准教授 (40533625)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | マイクロ流体デバイス / 肝細胞機能 / 肝小葉 / 肝細胞 |
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| Outline of Final Research Achievements |
During the drug development process, reproduction of the hepatic architecture and function in vitro is necessary and important to evaluate the efficacy and toxicity of the candidate drugs. In this study, we established two different types of oxygen gradient microfluidic device that mimics an in vitro hepatic lobular architecture.
After simulating the gradient of oxygen concentration, we evaluated the real oxygen concentration gradient in the devices using a sensing film coated with an oxygen-responsive dye. We also confirmed the survival and functions of hepatocytes that had been seeded inside the devices by using a live and dead cell staining kit and the indocyanine green uptake and excretion test, respectively.
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Report
(3 results)
Research Products
(53 results)
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[Journal Article] Identification of a novel alpha-fetoprotein- expressing cell population induced by Jagged1/Notch2 signal in murine fibrotic liver.2017
Author(s)
Yasuhiro Nakano, Sachie Nakao, Hideaki Sumiyoshi, Kenichiro Mikami, Yuri Tanno, Minako Sueoka, Daigo Kasahara, Hiroshi Kimura, Tadashi Moro, Akihide Kamiya, Katsuto Hozumi, Yutaka Inagaki
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Journal Title
Hepatol Commun
Volume: 印刷中
Issue: 3
Pages: 1-15
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] A combination of mitochondrial oxidative stress and excess fat/calorie intake accelerates steatohepatitis by enhancing hepatic CC chemokine production in mice.2016
Author(s)
Moro T, Nakao S, Sumiyoshi H, Ishii T, Miyazawa M, Ishii N, Sato T, Iida Y, Okada Y, Tanaka M, Hayashi H, Ueha S, Matsushima K, Inagaki Y
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Journal Title
PLoS ONE
Volume: 11
Issue: 1
Pages: e0146592-e0146592
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Mice overexpressing integrin αv in fibroblasts exhibit dermal thinning of the skin.2015
Author(s)
Wang Z, Jinnin M, Kobayashi Y, Kudo H, Inoue K, Nakayama W, Honda N, Makino K, Kajihara I, Makino T, Fukushima S, Inagaki Y, Ihn H.
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Journal Title
J Dermatol Sci.
Volume: 79
Issue: 3
Pages: 268-278
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Reduced supply of monocyte-derived macrophages leads to a transition from nodular to diffuse lesions and tissue cell activation in silica-induced pulmonary fibrosis in mice.2015
Author(s)
Shigeyuki Shichino, Jun Abe, Satoshi Ueha, Mikiya Otsuji, Tatsuya Tsukui, Mizuha Kosugi-Kanaya, Francis HW Shand, Shin-ichi Hashimoto, Hiroshi I. Suzuki, Teppei Morikawa, Yutaka Inagaki, and Kouji Matsushima:
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Journal Title
Am J Pathol
Volume: 185
Issue: 11
Pages: 2923-2938
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Presentation] Functional analysis of a novel regeneration factor, opioid growth factor receptor-like 1, that regulates both hepatic development and regeneration of injured/fibrotic liver.2016
Author(s)
Takayo Yanagawa, Hideaki Sumiyoshi, Yousuke Chiba, Yuhei Suzuki, Sachie Nakao, Hiromi Chikada, Akihide Kamiya, Yutaka Inagaki
Organizer
67th Annual Meeting of the American Association for the Study of Liver Diseases
Place of Presentation
Hynes Convention Center (Boston, MA, U.S.A.)
Year and Date
2016-11-14
Related Report
Int'l Joint Research
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[Presentation] Contribution of Jagged1/Notch signaling to murine fibrotic liver regeneration through possible dedifferentiation of mature hepatocytes.2016
Author(s)
Yasuhiro Nakano, Sachie Nakao, Hideaki Sumiyoshi, Kenichiro Mikami, Yuri Tanno, Daigo Kasahara, Hiroshi Kimura, Tadashi Moro, Akihide Kamiya, Katsuto Hozumi, Yutaka Inagaki
Organizer
67th Annual Meeting of the American Association for the Study of Liver Diseases
Place of Presentation
Hynes Convention Center (Boston, MA, U.S.A.)
Year and Date
2016-11-12
Related Report
Int'l Joint Research
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[Presentation] Contribution of Jagged1/Notch signaling to murine fibrotic liver regeneration through possible dedifferentiation of mature hepatocytes.2016
Author(s)
Yasuhiro Nakano, Sachie Nakao, Hideaki Sumiyoshi, Kenichiro Mikami, Yuri Tanno, Minako Sueoka, Daigo Kasahara, Hiroshi Kimura, Tadashi Moro, Akihide Kamiya, Katsuto Hozumi, Yutaka Inagaki
Organizer
9th Notch Meeting
Place of Presentation
国立遺伝学研究所（静岡県三島市）
Year and Date
2016-10-05
Related Report
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[Presentation] Opioid growth factor receptor-like 1; a novel bone marrow cells-derived factor that accelerates regeneration of murine fibrotic liver.2015
Author(s)
Takayo Yanagawa, Hideaki Sumiyoshi, Sachie Nakao, Masato Otsuka, Minoru Kimura, Yosuke Chiba, Shintaro Satake, Tadashi Moro, Hiromi Chikada, Akihide Kamiya, and Yutaka Inagaki:
Organizer
66th Annual Meeting of the American Association for the Study of Liver Diseases
Place of Presentation
Moscone West Convention Center (アメリカ合衆国カリフォルニア州）
Year and Date
2015-11-16
Related Report
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[Presentation] 肝線維化と再生の病態連繋．2015
Author(s)
稲垣 豊、住吉秀明
Organizer
第22回肝細胞研究会
Place of Presentation
米子コンベンションセンター（鳥取県米子市）
Year and Date
2015-06-04
Related Report
Invited
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