An immunoinhibitory receptor, Allergin-1 is the target molecule for treatment of allergic asthma

• Project/Area Number: 15K15319
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Respiratory organ internal medicine
• Research Institution: University of Tsukuba
• Principal Investigator: TAHARA Satoko 筑波大学, 生命領域学際研究センター, 講師 (20360589)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 喘息 / アラジン－１ / House dust mite / アレルギー性喘息 / House Dust Mite

Outline of Final Research Achievements

Allergin-1 is an inhibitory immunoglobulin-like receptor bearing immunoreceptor tyrosine-based inhibitory motif (ITIM) in its cytoplasmic region. Allergin-1 inhibits house dust mite-induced serum IgE elevation, airway eosinophilia, and airway hyperreactivity (AHR), therefore, Allergin-1 may be a target molecule for treatment of allergic asthma. In this proposal, a phospholipid liposome was used to treat the HDM-induced allergic asthma. Because, one of the phospholipid has an ability to bind to Allergin-1. However, in the HDM-induced asthma model, not only Allergin-1-binding phospholipid, but also phosphatidylcholine, which does not bind to Allergin-1 inhibited the AHR.

Research Products

• [Journal Article] Allergin-1 inhibits TLR2-mediated mast cell activation and suppresses dermatitis (2016)
  • Author(s): Tsurusaki S, Tahara-Hanaoka S, Shibagaki S, Miyake S, Imai M, Shibayama S, Kubo M, Shibuya A
  • Journal Title: International Immunology Volume: 28(12) Issue: 12 Pages: 605-609
  • DOI: 10.1093/intimm/dxw046
  • NAID: 120007135029
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Marginal zone B cells exacerbate endotoxic shock via interleukin-6 secretion induced by Fcα/μR-coupled TLR4 signalling (2016)
  • Author(s): Honda S, Sato K, Totsuka N, Fujiyama S, Fujimoto M, Miyake K, Nakahashi-Oda C, Tahara-Hanaoka S, Shibuya K, Shibuya A
  • Journal Title: Nat Commun Volume: 7 Issue: 1 Pages: 1-10
  • DOI: 10.1038/ncomms11498
  • NAID: 120007135503
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Apoptotic epithelial cells control the abundance of Treg cells at barrier surfaces (2016)
  • Author(s): Nakahashi-Oda C, Udayanga KGS, Nakamura Y, Nakazawa Y, Miki H, Iino S, Tahara-Hanaoka S, Shibuya K, Shibuya A.
  • Journal Title: Nature Immunol, 17(4):441-453, 2016 Volume: 17 Issue: 4 Pages: 441-453
  • DOI: 10.1038/ni.3345
  • Peer Reviewed / Open Access
• [Journal Article] Tie2 Signaling Enhances Mast Cell Progenitor Adhesion to Vascular Cell Adhesion Molecule-1 (VCAM-1) through α4β1 Integrin. (2015)
  • Author(s): Kanemaru K, Noguchi E, Tokunaga T, Nagai K, Hiroyama T, Nakamura Y, Tahara-Hanaoka S, Shibuya A.
  • Journal Title: PLoS One, 10(12), 2015 Volume: 10 Issue: 12 Pages: 1-16
  • DOI: 10.1371/journal.pone.0144436
  • NAID: 120007130029
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Identification and Characterization of CD300H, a New Member of the Human CD300 Immunoreceptor Family (2015)
  • Author(s): Niizuma K, Tahara-Hanaoka S, Noguchi E, Shibuya A.
  • Journal Title: J. Biol. Chem, 290(36):22298-22308, 2015 Volume: 290 Issue: 36 Pages: 22298-22308
  • DOI: 10.1074/jbc.m115.643361
  • NAID: 120007136146
  • Peer Reviewed / Open Access
• [Journal Article] CD155 (PVR/Necl5) Mediates a Costimulatory Signal in CD4+ T Cells and Regulates Allergic Inflammation (2015)
  • Author(s): 4.Yamashita--Kanemaru Y, Takahashi Y, Wang Y, Tahara-Hanaoka S, Honda S, Bernhardt G, Shibuya A, Shibuya K.
  • Journal Title: J Immunol, 194(12):5644-5653, 2015 Volume: 194 Issue: 12 Pages: 5644-5653
  • DOI: 10.4049/jimmunol.1401942
  • Peer Reviewed / Open Access
• [Presentation] Clec10a, suppresses Der f-induced immune response and dermatitis (2016)
  • Author(s): Kanemaru K, Denda-Nagai K, Irimura T, Tahara-Hanaoka S, Shibuya A. .
  • Organizer: 第45回日本免疫学会総会・学術集会
  • Place of Presentation: 沖縄コンベンションセンター（沖縄県宜野湾市）
  • Year and Date: 2016-12-07
• [Presentation] Characterization of mouse ES cell-derived mast cell line, MEDMC-BRC6. (2016)
  • Author(s): Shibagaki S, Tahara-Hanaoka S, Shibuya A.
  • Organizer: 第45回日本免疫学会総会・学術集会
  • Place of Presentation: 沖縄コンベンションセンター（沖縄県宜野湾市）
  • Year and Date: 2016-12-06
• [Presentation] llergin-1 suppresses Toll-like receptor 2 ligand-induced dermatitis in mice (2015)
  • Author(s): Tsurusaki S, Tahara-Hanaoka S, Shibayama S, Shibuya A.
  • Organizer: 第44回日本免疫学会総会・学術集会
  • Place of Presentation: 札幌コンベンションセンター（北海道）
  • Year and Date: 2015-11-20
• [Presentation] Tie2 Signaling Enhances Mast Cell Progenitor Adhesion to VCAM-1 through β1 Integrin (2015)
  • Author(s): Kanemaru K, Noguchi E, Nagai K, Tahara-Hanaoka S, Shibuya A
  • Organizer: 第44回日本免疫学会総会・学術集会
  • Place of Presentation: 札幌コンベンションセンター（北海道）
  • Year and Date: 2015-11-18
• [Presentation] Allergin-1 in House Dust Mite-induced Allergic Asthma Model. (2015)
  • Author(s): Tahara-Hanaoka S, Hitomi K, Niizuma K, Shibagaki S, Miki H, Kubo M, Shibuya A.
  • Organizer: International Symposium on Immune Regulation,
  • Place of Presentation: Oarai Park Hotel, Ibaraki
  • Year and Date: 2015-10-30
• [Remarks] 筑波大学 免疫学研究室
  • URL: http://immuno-tsukuba.com/index.html