| Project/Area Number |
15K15320
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | Niigata University |
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Principal Investigator |
SAIJO YASUO 新潟大学, 医歯学系, 教授 (10270828)
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| Co-Investigator(Kenkyū-buntansha) |
笹岡 俊邦 新潟大学, 脳研究所, 教授 (50222005)
小田 佳奈子 新潟大学, 脳研究所, 特任助教 (60708212)
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| Co-Investigator(Renkei-kenkyūsha) |
SAKIMURA KENJI 新潟大学, 脳研究所, 教授 (40162325)
OHUCHI HIDEYO 岡山大学, 医歯(薬)学総合研究科, 教授 (00253229)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 肺再生 / 胚盤胞補完法 / 多能性幹細胞 / fgf10 / iPS細胞 |
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| Outline of Final Research Achievements |
Ｗe have developed Fgf10-/- mice by CRISPR/Cas system . Since the Fgf10-/- die immediately after birth, the Fgf10+/- genotype mice are maintained. We implanted 40 control embryos that was not injected ES cells into pseudopregnant mice and we obtained 13 littermates (32.5%) from the mice. 4 littermates (30.7%) showed limb defect (Fgf10-/-) and they were confirmed histologically that they had hypoplastic or aplastic lung. EGFPposotive ES cells were injected into blacystocyte of Fgf10-/- mice. 45 littermates were obtained from those mice. 38 littermates were EGFP positive. All EGFP positive mice showed the lung organ histologically. We sacrificed and analyzed histology in EGFP positive mice over one month. EGFP was positive in their lungs. The tissue, cells, and structure of their lung were well developed and not different from normal mice lung by H-E staining. Frozen section of the lung showed EGFP positive in alveolar epithelium, endothelium, tracheal cartilage.
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