The challenge for the study of unmyelinated fibers in central nervous system

• Project/Area Number: 15K15342
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Neurology
• Research Institution: Doshisha University
• Principal Investigator: Nukina Nobuyuki 同志社大学, 脳科学研究科, 教授 (10134595)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2015: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
• Keywords: 無髄神経 / Nav1.2 / 有髄神経 / 無髄線維 / 有髄線維 / Nav2.1

Outline of Final Research Achievements

Unmyelinated fibers in central nervous systems(CNS) are not well known. Recently, we found Nav 1.2 localizes on the axons of unmyelinated fibers diffusely, not as concentrated localization in the Ranvier nodes on myelinated fibers. Based on this property, we investigated the localization of unmyelinated fibers in the CNS of mice. We found Nav 1.2 diffuse localization on the axons of mossy fibers in hippocampus, parallel fibers in cerebellum, and projection fibers of striatum which are already know to be unmyelinated. In addition to those fibers, we found diffuse localization of Nav 1.2 on the axons in stria terminals and some part of corpus callosum, We also found unmyelinated fibers by electron microscopy. The results suggest, there are still unknown or uninvestigated unmyeilated fibers in CNS.

Research Products

• [Int'l Joint Research] INSERM,France(フランス)
• [Int'l Joint Research] Washington University(米国)
• [Journal Article] Proteomic analysis of native cerebellar iFGF14 complexes. (2016)
  • Author(s): Bosch MK, Nerbonne JM, Townsend RR, Miyazaki H, Nukina N, Ornitz DM, Marionneau C.
  • Journal Title: Channels (Austin). Volume: 10 Issue: 4 Pages: 297-312
  • DOI: 10.1080/19336950.2016.1153203
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Splicing misregulation of SCN5A contributes to cardiac-conduction delay and heart arrhythmia in myotonic dystrophy. (2016)
  • Author(s): Freyermuth F, Rau F, Kokunai Y, Linke T, Sellier C, Nakamori M, Kino Y,.......Zimmer T, Furling D, Takahashi MP, Charlet-Berguerand N.
  • Journal Title: Nat Commun. Volume: 7 Issue: 1 Pages: 11067-11067
  • DOI: 10.1038/ncomms11067
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Structure-based site-directed photo-crosslinking analyses of multimeric cell-adhesive interactions of voltage-gated sodium channel β subunits. (2016)
  • Author(s): Shimizu H, Miyazaki H, Ohsawa N, Shoji S, Ishizuka-Katsura Y, Tosaki A, Oyama F, Terada T, Sakamoto K, Shirouzu M, Sekine S, Nukina N, Yokoyama S.
  • Journal Title: Sci Rep. Volume: 6 Issue: 1
  • DOI: 10.1038/srep26618
  • Peer Reviewed / Open Access
• [Journal Article] Genome-wide analyses in neuronal cells reveal that upstream transcription factors regulate lysosomal gene expression. (2016)
  • Author(s): Yamanaka T, Tosaki A, Kurosawa M, Shimogori T, Hattori N, Nukina N
  • Journal Title: FEBS J Volume: 283(6) Issue: 6 Pages: 1077-87
  • DOI: 10.1111/febs.13650
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] erine 403-phosphorylated p62/SQSTM1 immunoreactivity in inclusions of neurodegenerative diseases (2015)
  • Author(s): Kurosawa,M., Matsumoto,G., Sumikura,H., Hatsuta,H., Murayama,S., Sakurai,T., Shimogori,T., Hattori,N. and Nukina,N.
  • Journal Title: Neurosci. Res. Volume: 103 Pages: 64-70
  • DOI: 10.1016/j.neures.2015.08.002
  • Peer Reviewed / Open Access
• [Journal Article] TBK1 controls autophagosomal engulfment of polyubiquitinated mitochondria through p62/SQSTM1 phosphorylation. (2015)
  • Author(s): Matsumoto G, Shimogori T, Hattori N, Nukina N.
  • Journal Title: Hum Mol Genet. Volume: 24 Issue: 15 Pages: 4429-42
  • DOI: 10.1093/hmg/ddv179
  • Peer Reviewed
• [Presentation] 大脳基底核疾患研究の病態脳科学的展開 (2016)
  • Author(s): 貫名信行
  • Organizer: 第５７回日本神経学会学術大会(楢林賞受賞講演）
  • Place of Presentation: 神戸コンベンションセンター（兵庫、神戸）
  • Year and Date: 2016-05-19
  • Invited
• [Remarks] 同志社大学大学院脳科学研究科認知記憶加齢部門
  • URL: http://brainscience.doshisha.ac.jp/introduction/pat/ca.html
• [Remarks] 同志社大学大学院脳科学研究科貫名研究室
  • URL: http://snp.hippy.jp/