Identification of novel glucagon-related peptides by using C-terminal anti-glucagon antibody

Research Project

Project/Area Number	15K15345
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	Metabolomics
Research Institution	Gunma University
Principal Investigator	KITAMURA TADAHIRO 群馬大学, 生体調節研究所, 教授 (20447262)
Project Period (FY)	2015-04-01 – 2017-03-31
Project Status	Completed (Fiscal Year 2016)
Budget Amount *help	¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000) Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000) Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords	グルカゴン / 糖尿病
Outline of Final Research Achievements	We tried to identify novel glucagon-related peptides by using affinity chromatography with C-terminal anti-glucagon antibody and mouse plasma samples. However, due to insufficient sample volume, we failed to identify such molecules. Then we are now trying to do it using mouse pancreas or intestine. We also evaluated currently commercially available immunoassays for glucagon by comparing with our newly developed LC/MS/MS system and found that Mercodia sandwich ELISA has much higher correlation with LC/MS/MS compared to conventional RIA.