| Project/Area Number |
15K15364
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Hematology
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| Research Institution | Kyushu University |
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Principal Investigator |
Kunisaki Yuya 九州大学, 医学研究院, 助教 (80737099)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 造血幹細胞 / 骨髄微小環境 / がん幹細胞 / 生体イメージング / 骨髄環境 / 白血病 / 光化学技術 / 交感神経 |
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| Outline of Final Research Achievements |
We propose to stimulate peripheral sympathetic nerves with a novel optogenetic model, and investigate the transmission of neural signals in the BM by imaging the release of intracellular Ca2+ in different stromal cell populations with multi-channel fluorescent intravital microscopy. Calcium (Ca2+) is a universal second messenger regulating essential cellular signaling events in many tissues and cell types. Ca2+ flux in a cellular level can be imaged in vivo using an enhanced green fluorescent protein (EGFP) based, genetically encoded Ca2+ indicator, GCaMP3, which provides highest signal-to-noise ratio (SNR) up to date. In thisstudy, using genetic mouse models that express GCaMP in endothelial cells (VE-cadherinERT2), we will establish the imaging system to visualize signal transmissions in the bone marrow endothelial cells under sympathetic stimulation using optogenetic approaches combined with BM multichannel fluorescence intravital microscopy (MFIM).
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