Mechanisms by which sympathetic nerves regulate functions of hematopoietic stem cell niches by in vivo imaging techniques
Project/Area Number |
15K15364
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Hematology
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Research Institution | Kyushu University |
Principal Investigator |
Kunisaki Yuya 九州大学, 医学研究院, 助教 (80737099)
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Project Period (FY) |
2015-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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Keywords | 造血幹細胞 / 骨髄微小環境 / がん幹細胞 / 生体イメージング / 骨髄環境 / 白血病 / 光化学技術 / 交感神経 |
Outline of Final Research Achievements |
We propose to stimulate peripheral sympathetic nerves with a novel optogenetic model, and investigate the transmission of neural signals in the BM by imaging the release of intracellular Ca2+ in different stromal cell populations with multi-channel fluorescent intravital microscopy. Calcium (Ca2+) is a universal second messenger regulating essential cellular signaling events in many tissues and cell types. Ca2+ flux in a cellular level can be imaged in vivo using an enhanced green fluorescent protein (EGFP) based, genetically encoded Ca2+ indicator, GCaMP3, which provides highest signal-to-noise ratio (SNR) up to date. In thisstudy, using genetic mouse models that express GCaMP in endothelial cells (VE-cadherinERT2), we will establish the imaging system to visualize signal transmissions in the bone marrow endothelial cells under sympathetic stimulation using optogenetic approaches combined with BM multichannel fluorescence intravital microscopy (MFIM).
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Report
(3 results)
Research Products
(15 results)
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[Journal Article] Identification of unipotent megakaryocyte progenitors in human hematopoiesis.2017
Author(s)
K. Miyawaki. Iwasaki, T. Jiromaru, H. Kusumoto, A. Yurino, T. Sugio, Y. Uehara, J. Odawara, S. Daitoku, Y. Kunisaki, Y. Mori, Y. Arinobu, H. Tsuzuki, Y. Kikushige, T. Iino, K. Kato, K. Takenaka, T. Miyamoto, T. Maeda, *K. Akashi
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Journal Title
Blood
Volume: 印刷中
Issue: 25
Pages: 3332-3343
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Enhanced Reconstitution of Human Erythropoiesis and Thrombopoiesis in an Immunodeficient Mouse Model with Kit(Wv) Mutations.2016
Author(s)
A. Yurino, K. Takenaka, T. Yamauchi, T. Nunomura, Y. Uehara, F. Jinnouchi, K. Miyawaki, Y. Kikushige, K. Kato, T. Miyamoto, H. Iwasaki, Y. Kunisaki, *K. Akashi
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Journal Title
Stem Cell Reports
Volume: 7
Issue: 3
Pages: 425-438
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Fetal liver hematopoietic stem cell niches associate with portal vessels2016
Author(s)
Khan JA, Mendelson A, Kunisaki Y, Birbrair A, Kou Y, Arnal-Estapé A, Pinho S, Ciero P, Nakahara F, Ma'ayan A, Bergman A, Merad M, Frenette PS.
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Journal Title
Science
Volume: 351
Issue: 6269
Pages: 176-180
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Neutrophil ageing is regulated by the microbiome2015
Author(s)
Zhang D, Chen G, Manwani D, Mortha A, Xu C, Faith JJ, Burk RD, Kunisaki Y, Jang JE, Scheiermann C, Merad M, Frenette PS.
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Journal Title
Nature
Volume: 525
Issue: 7570
Pages: 528-532
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Presentation] 造血幹細胞ニッチ2015
Author(s)
國崎 祐哉
Organizer
第77回日本血液学会学術集会
Place of Presentation
金沢
Year and Date
2015-10-15
Related Report
Invited
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