Cancer stem cell assay by a new immunodeficient mouse model Cancer stem cell assay by a new immunodeficient mouse model harboring with optimal bone marrow microenvironment
Project/Area Number |
15K15365
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Hematology
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Research Institution | Kyushu University |
Principal Investigator |
Akashi Koichi 九州大学, 医学研究院, 教授 (80380385)
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Project Period (FY) |
2015-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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Keywords | がん幹細胞 / 異種移植アッセイ / 免疫不全マウス / マクロファージ / SIRPA / 骨髄ニッチ / ヒト疾患モデル / マクロファージ寛容 / 幹細胞アッセイシステム / 異種移植モデル |
Outline of Final Research Achievements |
To evaluate human cancer stem cells in xenotransplantation models, a variety of immunodeficient mouse lines have been developed. We found that in xenograft engraftment, inhibition of phagocytic reaction of mouse macrophages is essential in addition to lymphoid depletion. Here, we have established a new immunodeficient BRGhS mouse line, harbouring a complete macrophage tolerance. Furthermore, we introduced KITW/v mutation into BRGhS line to optimize mouse bone marrow microenvironment for human cell engraftment. This strain should be very useful for human cancer stem cell assay.
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Report
(4 results)
Research Products
(33 results)
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[Journal Article] Functional interleukin-33 receptors are expressed in early progenitor stages of allergy-related granulocytes.2017
Author(s)
Tsuzuki H, Arinobu Y, Miyawaki K, Takaki A, Ota SI, Ota Y, Mitoma H, Akahoshi M, Mori Y, Iwasaki H, Niiro H, Tsukamoto H, Akashi K.
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Journal Title
Immunology
Volume: 150
Pages: 64-73
DOI
Related Report
Peer Reviewed
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[Journal Article] Calreticulin mutation does not contribute to disease progression in essential thrombocythemia by inhibiting phagocytosis.2016
Author(s)
Daitoku S, Takenaka K, Yamauchi T, Yurino A, Jinnouchi F, Nunomura T, Eto T, Kamimura T, Higuchi M, Harada N, Saito N, Miyamoto T, Iwasaki H, Akashi K.
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Journal Title
Exp Hematol
Volume: 44
Pages: 817-825
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Enhanced Reconstitution of Human Erythropoiesis and Thrombopoiesis in an Immunodeficient Mouse Model with KitWv Mutations.2016
Author(s)
Yurino A, Takenaka K, Yamauchi T, Nunomura T, Uehara Y, Jinnouchi F, Miyawaki K, Kikushige Y, Kato K, Miyamoto T, Iwasaki H, Kunisaki Y, Akashi K.
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Journal Title
Stem Cell Reports
Volume: 13
Pages: 425-438
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Clinical features and outcomes of patients with primary myelofibrosis in Japan: report of a 17-year nationwide survey by the Idiopathic Disorders of Hematopoietic Organs Research Committee of Japan.2016
Author(s)
Takenaka K, Shimoda K, Uchida N, Shimomura T, Nagafuji K, Kondo T, Shibayama H, Mori T, Usuki K, Azuma T, Tsutsumi Y, Tanaka J, Dairaku H, Matsuo K, Ozawa K, Kurokawa M, Arai S, Akashi K.
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Journal Title
Int J Hematol
Volume: 105
Pages: 59-69
DOI
Related Report
Peer Reviewed
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[Journal Article] Efficacy and safety analysis of chemotherapy for advanced colitis-associated colorectal cancer in Japan.2016
Author(s)
Nio K, Higashi D, Kumagai H, Arita S, Shirakawa T, Nakashima K, Shibata Y, Esaki M, Manabe T, Nagai S, Ueki T, Nakano M, Ariyama H, Kusaba H, Hirahashi M, Oda Y, Esaki T, Mitsugi K, Futami K, Akashi K, Baba E.
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Journal Title
Anticancer Drugs
Volume: 27
Pages: 457-463
DOI
Related Report
Peer Reviewed
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[Journal Article] Reduced Dose of Salvage-line Regorafenib Monotherapy for Metastatic Colorectal Cancer in Japan.2015
Author(s)
Hirano G, Makiyama A, Makiyama C, Esaki T, Oda H, Uchino K, Komoda M, Tanaka R, Matsushita Y, Mitsugi K, Shibata Y, Kumagai H, Arita S, Ariyama H, Kusaba H, Akashi K, Baba E.
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Journal Title
Anticancer Res
Volume: 35
Pages: 371-377
Related Report
Peer Reviewed
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[Journal Article] Clinical outcomes of a novel therapeutic vaccine with Tax peptide-pulsed dendritic cells for adult T cell leukaemia/lymphoma in a pilot study.2015
Author(s)
Suehiro Y, Hasegawa A, Iino T, Sasada A, Watanabe N, Matsuoka M, Takamori A, Tanosaki R, Utsunomiya A, Choi I, Fukuda T, Miura O, Takaishi S, Teshima T, Akashi K, Kannagi M, Uike N, Okamura J.
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Journal Title
Br J Haematol
Volume: 169
Pages: 356-367
DOI
Related Report
Peer Reviewed
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[Journal Article] A multinational, open-label, phase 2 study of ruxolitinib in Asian patients with myelofibrosis: Japanese subset analysis.2015
Author(s)
Oritani K, Okamoto S, Tauchi T, Saito S, Ohishi K, Handa H, Takenaka K, Gopalakrishna P, Amagasaki T, Ito K, Akashi K.
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Journal Title
Int J Hematol
Volume: 101
Pages: 295-304
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Factors prognostic of eligibility for allogeneic HCT among older patients with AML-CR1 and adverse- or intermediate-risk cytogenetics.2015
Author(s)
Yamasaki S, Yoshimoto G, Ogawa R, Aoki K, Higuchi M, Harada N, Arima F, Kondo S, Matubara F, Takahashi T, Uike N, Miyamoto T, Okamura S, Akashi K.
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Journal Title
Ann Hematol
Volume: 94
Pages: 1159-1165
DOI
Related Report
Peer Reviewed
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[Journal Article] α-Mannan induces Th17-mediated pulmonary graft-versus-host disease in mice.2015
Author(s)
Uryu H, Hashimoto D, Kato K, Hayase E, Matsuoka S, Ogasawara R, Takahashi S, Maeda Y, Iwasaki H, Miyamoto T, Saijo S, Iwakura Y, Hill GR, Akashi K, Teshima T.
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Journal Title
Blood
Volume: 125
Pages: 3014-3023
DOI
Related Report
Peer Reviewed
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[Journal Article] Reduced Tyk2 gene expression in β-cells due to natural mutation determines susceptibility to virus-induced diabetes.2015
Author(s)
Izumi K, Mine K, Inoue Y, Teshima M, Ogawa S, Kai Y, Kurafuji T, Hirakawa K, Miyakawa D, Ikeda H, Inada A, Hara M, Yamada H, Akashi K, Niho Y, Ina K, Kobayashi T, Yoshikai Y, Anzai K, Yamashita T, Minagawa H, Fujimoto S, Kurisaki H, Shimoda K, Katsuta H, Nagafuchi S.
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Journal Title
Nat Commun
Volume: 6
Pages: 6748
DOI
Related Report
Peer Reviewed
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[Journal Article] HLA-Haploidentical Peripheral Blood Stem Cell Transplantation with Post-Transplant Cyclophosphamide after Busulfan-Containing Reduced-Intensity Conditioning.2015
Author(s)
Sugita J, Kawashima N, Fujisaki T, Kakihana K, Ota S, Matsuo K, Miyamoto T, Akashi K, Taniguchi S, Harada M, Teshima T.
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Journal Title
Biol Blood Marrow Transplant
Volume: 21
Pages: 1646-1652
DOI
Related Report
Peer Reviewed
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[Journal Article] Increased CD226 Expression on CD8+ T Cells Is Associated with Upregulated Cytokine Production and Endothelial Cell Injury in Patients with Systemic Sclerosis.2015
Author(s)
Ayano M, Tsukamoto H, Kohno K, Ueda N, Tanaka A, Mitoma H, Akahoshi M, Arinobu Y, Niiro H, Horiuchi T, Akashi K.
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Journal Title
J Immunol
Volume: 195
Pages: 892-900
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Expression of programmed cell death ligand 1 is associated with poor overall survival in patients with diffuse large B-cell lymphoma.2015
Author(s)
Kiyasu J, Miyoshi H, Hirata A, Arakawa F, Ichikawa A, Niino D, Sugita Y, Yufu Y, Choi I, Abe Y, Uike N, Nagafuji K, Okamura T, Akashi K, Takayanagi R, Shiratsuchi M, Ohshima K.
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Journal Title
Blood
Volume: 126
Pages: 2193-2201
DOI
Related Report
Peer Reviewed
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[Journal Article] A TIM-3/Gal-9 Autocrine Stimulatory Loop Drives Self-Renewal of Human Myeloid Leukemia Stem Cells and Leukemic Progression.2015
Author(s)
Kikushige Y, Miyamoto T, Yuda J, Jabbarzadeh-Tabrizi S, Shima T, Takayanagi S, Niiro H, Yurino A, Miyawaki K, Takenaka K, Iwasaki H, Akashi K.
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Journal Title
Cell Stem Cell
Volume: 17
Pages: 341-352
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Chd5 Regulates MuERV-L/MERVL Expression in Mouse Embryonic Stem Cells Via H3K27me3 Modification and Histone H3.1/H3.2.2015
Author(s)
Hayashi M, Maehara K, Harada A, Semba Y, Kudo K, Takahashi H, Oki S, Meno C, Ichiyanagi K, Akashi K, Ohkawa Y.
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Journal Title
J Cell Biochem
Volume: 117
Pages: 780-792
DOI
Related Report
Peer Reviewed
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