Title Dilution effect of the tumor derived exsome

• Project/Area Number: 15K15369
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Hematology
• Research Institution: Tokai University
• Principal Investigator: KOTANI AI 東海大学, 医学部, 教授 (00517477)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2016: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
• Keywords: エクソソーム

Outline of Final Research Achievements

In previous study, it has been shown that the tumor derived exosome, a kind of extracellular vesicle, critically works for development of EBV+ lymphoproliferative diseases (LPD). Thereafter, in order to evaluate the efficacy of the depletion of exosome in these diseases, we tested whether the dilution of the tumor derived exosome effect on the tumor development by use of "parabiosis". As a results, the pair of mouse with and without caner, unexpectedly died just a week after parabiosis, while that of mice with cancer and that of mice without caner survived much longer than that. The mechanistic analysis of the result will be studied.

Research Products

• [Journal Article] Quantitation of circulating satellite RNAs in pancreatic cancer patients (2016)
  • Author(s): Kishikawa T, Otsuka M, Yoshikawa T, Ohno M, Yamamoto K, Yamamoto N, Kotani A, Koike K
  • Journal Title: JCI Insight Volume: 1(8) Issue: 8
  • DOI: 10.1172/jci.insight.86646
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] miR-133 regulates Evi1 expression in AML cells as a potential therapeutic target. (2016)
  • Author(s): Yamamoto H, Lu J, Oba S, Kawamata T, Yoshimi A, Kurosaki N, Yokoyama K, Matsushita H, Kurokawa M, Tojo A, Ando K, Morishita K, Katagiri K, Kotani A
  • Journal Title: Scientific Reports Volume: 12 Issue: 1 Pages: 10294-10294
  • DOI: 10.1038/srep19204
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Pathogenesis and novel therapy for EBV-related B-cell lymphoma (2016)
  • Author(s): Sato A, Yamakawa N, Kotani A.
  • Journal Title: Rinsho Ketsueki Volume: 57 Issue: 1 Pages: 3-8
  • DOI: 10.11406/rinketsu.57.3
  • NAID: 130005125835
  • ISSN: 0485-1439, 1882-0824
  • Peer Reviewed / Int'l Joint Research
• [Presentation] 転写因子欠損下におけるmiRNAによる細胞運命制御 (2016)
  • Author(s): Kotani A
  • Organizer: 第39回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-11-30
  • Invited
• [Presentation] EBV Establishes the Tumor Microenvironment by Exosome-mediated Delivery of miRNA to Macrophages (2016)
  • Author(s): Kotani A
  • Organizer: 57th Autumn Meeting of the Korean Society of Hematology
  • Place of Presentation: Daejeon Convention Center（韓国・大田広域市）
  • Year and Date: 2016-11-11
  • Int'l Joint Research / Invited
• [Presentation] A single miRNAs rescue transcriptional factor deficiency in B cell lineage specification though TGF beta pathway (2016)
  • Author(s): Kotani A
  • Organizer: 第78回日本血液学会学術集会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-10-13
  • Invited
• [Presentation] EBV establishes the tumor microenvironment by exosome-mediated delivery of miRNA to macrophages (2016)
  • Author(s): Kotani A
  • Organizer: 第75回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-10-06
  • Invited
• [Presentation] 転写因子欠損を補完するmiRNAによる造血運命決定機構と白血病の新しい理解 (2016)
  • Author(s): Kotani A
  • Organizer: 第89回日本生化学会大会
  • Place of Presentation: 仙台国際センター／ 東北大学川内北キャンパス（宮城県仙台市）
  • Year and Date: 2016-09-25
  • Invited
• [Presentation] EBV establishes the tumor microenvironment by exosome-mediated delivery of miRNA to macrophages (2016)
  • Author(s): Kotani A
  • Organizer: 2nd Bio-iST Symposium 2016
  • Place of Presentation: マレーシア日本国際工科院（マレーシア・クアラルンプール）
  • Int'l Joint Research / Invited
• [Presentation] 転写因子欠損下でもmiRNAはB細胞の運命を決定する (2015)
  • Author(s): 幸谷 愛
  • Organizer: 第74回日本癌学会
  • Place of Presentation: 名古屋国際会議場 (愛知県 名古屋市)
  • Year and Date: 2015-10-08
  • Int'l Joint Research / Invited
• [Book] パラダイムシフトをもたらすエクソソーム機能研究最前線 (2017)
  • Author(s): 落谷 孝広, 松田 大介, 原田 結, 芝 清隆, 高橋 有己, 西川 元也, 高倉 喜信, 遠藤 恒介, 田原 栄治, 田原 栄俊, 下田 麻子, 秋吉 一成, 瀧澤 俊広, 大口 昭英, 竹下 俊行, 齋藤 滋, 大塚 蔵嵩, 河原 裕憲, 仲谷 利栄, 幸谷 愛, et al.
  • Total Pages: 314
  • Publisher: エヌ・ティー・エス
• [Book] Chronic Inflammation Mechanisms and Regulation (2016)
  • Author(s): Aoki T, Narumiya S, Tsukui, T, Shichino S, Shimaoka T, Ueha S, Matsushima K, Ito D, Kumanogoh A, Ito Y, Mokuda S, Miyata K, Matsushima T, Asahara H, Yasutomo K, Taniguchi R, Nureki O, Arita M, Sasaki J, Kotani A, et al.
  • Total Pages: 702
  • Publisher: Springer Japan
• [Remarks] 幸谷研究室
  • URL: http://kotani.med.u-tokai.ac.jp/
• [Remarks] JSTさきがけ慢性炎症領域研究者紹介
  • URL: http://inflam.jst.go.jp/research/p_2404kotani/index.html