Identification of chitin binding protein(s) and analysis of cellular signal transduction by stimulation of chitin.

• Project/Area Number: 15K15377
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Collagenous pathology/Allergology
• Research Institution: National Center for Child Health and Development
• Principal Investigator: Saito Hirohisa 国立研究開発法人国立成育医療研究センター, 研究所, 副所長 (40130166)
• Co-Investigator(Renkei-kenkyūsha): NAKAE SUSUMU 東京大学, 医科学研究所 システムズバイオロジー分野, 准教授 (60450409) ; SUDO KATSUKO 東京医科大学, 動物実験センター, 講師 (50126091) ; MATSUMOTO KENJI 国立研究開発法人国立成育医療研究センター, 研究所 免疫アレルギー・感染研究部, 部長 (60181765) ; NAKAJIMA HIDEKI 国立研究開発法人国立成育医療研究センター, 研究所 マススクリーニング研究室, 研究員 (30450620)
• Project Period (FY): 2015-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2015: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
• Keywords: アレルギー / キチン / Th2

Outline of Final Research Achievements

Although it is considered that chitin (a major component of the outer shell of house dust mite) can contribute to the induction of allergic responses, it still remains unclear the mechanism of cellular signal transduction by stimulation of chitin. In this study, we attempted to identify chitin binding proteins, and finally found four chitin-binding proteins. In the future, we will investigate the role of these molecules in development of house dust mite antigen-mediated allergic disorders.

Research Products

• [Int'l Joint Research] SIAF(Switzerland)
• [Int'l Joint Research] Stanford University School of Medicine/Departments of Pathology(米国)
• [Int'l Joint Research] Cambridge/MRC Laboratory of Molecular Biology(英国)
• [Journal Article] Platelets constitutively express IL-33 protein and modulate eosinophilic airway inflammation. (2016)
  • Author(s): Takeda T, Unno H, Morita H, Futamura K, Emi-Sugie M, Arae K, Shoda T, Okada N, Igarashi A, Inoue E, Kitazawa H, Nakae S, Saito H, Matsumoto K, Matsuda A.
  • Journal Title: J Allergy Clin Immunol Volume: In Press Issue: 5 Pages: 1395-1403
  • DOI: 10.1016/j.jaci.2016.01.032
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Sera of patients with infantile eosinophilic gastroenteritis showed a specific increase in both thymic stromal lymphopoietin and IL-33 levels (2016)
  • Author(s): Shoda T, Matsuda A, Arai K, Shimizu H, Morita H, Orihara K, Okada N, Narita M, Ohya Y, Saito H, Matsumoto K, Nomura I.
  • Journal Title: J Allergy Clin Immunol. Volume: in press Issue: 1 Pages: 299-303
  • DOI: 10.1016/j.jaci.2015.11.042
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Recent advances in understanding the roles of vascular endothelial cells in allergic inflammation (2016)
  • Author(s): Shoda T, Futamura K, Orihara K, Emi-Sugie M, Saito H, Matsumoto K, Matsuda A.
  • Journal Title: Allergology International Volume: 65 Issue: 1 Pages: 21-29
  • DOI: 10.1016/j.alit.2015.08.001
  • NAID: 130005290335
  • ISSN: 1323-8930, 1440-1592
  • Peer Reviewed / Open Access
• [Journal Article] Transepidermal water loss measurement during infancy can predict the subsequent development of atopic dermatitis regardless of filaggrin mutations (2016)
  • Author(s): Horimukai K, Morita K, Narita M, Kondo M, Kabashima S, Inoue E, Sasaki T, Niizeki H, Saito H, Matsumoto K, Ohya Y.
  • Journal Title: Allergology International Volume: 65 Issue: 1 Pages: 103-108
  • DOI: 10.1016/j.alit.2015.09.004
  • NAID: 130005290309
  • ISSN: 1323-8930, 1440-1592
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] IL-25 and IL-33 Contribute to Development of Eosinophilic Airway Inflammation in Epicutaneously Antigen-Sensitized Mice. (2015)
  • Author(s): Morita H, Arae K, Unno H, Toyama S, Motomura K, Matsuda A, Suto H, Okumura K, Sudo K, Takahashi T, Saito H, Matsumoto K, Nakae S.
  • Journal Title: PLoS One. Volume: 10 Issue: 7 Pages: e0134226-e0134226
  • DOI: 10.1371/journal.pone.0134226
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] An Interleukin-33-Mast Cell-Interleukin-2 Axis Suppresses Papain-Induced Allergic Inflammation by Promoting Regulatory T Cell Numbers. (2015)
  • Author(s): Morita H, Arae K, Unno H, Miyauchi K, Toyama S, Nambu A, Oboki K, Ohno T, Motomura K, Matsuda A, Yamaguchi S, Narushima S, Kajiwara N, Iikura M, Suto H, McKenzie AN, Takahashi T, Karasuyama H, Okumura K, Azuma M, Moro K, Akdis CA, Galli SJ, Koyasu S, Kubo M, Sudo K, Saito H, Matsumoto K, Nakae S.
  • Journal Title: Immunity Volume: 43 Issue: 1 Pages: 175-86
  • DOI: 10.1016/j.immuni.2015.06.021
  • NAID: 40020962854
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Measurement of allergen-specific secretory IgA in stool of neonates, infants and toddlers by protection against degradation of immunoglobulins and allergens (2015)
  • Author(s): Norio Kamemura, Miwa Takashima, Hideaki Morita, Kenji Matsumoto, Hiroshi Saito, and Hiroshi Kido.
  • Journal Title: The Journal of Medical Investigation Volume: 62 Issue: 3.4 Pages: 137-144
  • DOI: 10.2152/jmi.62.137
  • NAID: 130005099155
  • ISSN: 1343-1420, 1349-6867
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Total serum IgE level influences oral food challenge tests for IgE-mediated food allergies. (2015)
  • Author(s): Horimukai K, Hayashi K, Tsumura Y, Nomura I, Narita M, Ohya Y, Saito H, Matsumoto K.
  • Journal Title: Allergy Volume: 70 Issue: 3 Pages: 334-7
  • DOI: 10.1111/all.12562
  • Peer Reviewed
• [Journal Article] Global DNA hypomethylation coupled to cellular transformation and metastatic ability. (2015)
  • Author(s): Funaki S, Nakamura T, Nakatani T, Umehara H, Nakashima H, Okumura M, Oboki K, Matsumoto K, Saito H, Nakano T.
  • Journal Title: FEBS lett. Volume: 589 Issue: 24PartB Pages: 4053-60
  • DOI: 10.1016/j.febslet.2015.11.020
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Identification of a polyI:C-inducible membrane protein that participates in dendritic cell-mediated natural killer cell activation. (2015)
  • Author(s): Ebihara T, Azuma M, Oshiumi H, Kasamatsu J, Iwabuchi K, Matsumoto K, Saito H, Taniguchi T, Matsumoto M, Seya T.
  • Journal Title: J Exp Med Volume: 212 Issue: 8 Pages: 1337-1337
  • DOI: 10.1084/jem.2009157307012015c
  • NAID: 120002722561
  • Peer Reviewed