Using zebrafish for screening of Diamond-Blackfan anemia drugs
| Project/Area Number |
15K15394
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pediatrics
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| Research Institution | University of Miyazaki |
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Principal Investigator |
Yoshihama Maki 宮崎大学, フロンティア科学実験総合センター, 研究員 (00381103)
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| Co-Investigator(Kenkyū-buntansha) |
上地 珠代 宮崎大学, フロンティア科学実験総合センター, 研究員 (10381104)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 創薬 / ゼブラフィッシュ / バイオラベリング / 造血効果 / 毒性試験 / 薬物代謝 / 化合物スクリーニング / リボソーム / 疾患モデル / ゼブラフィッシュ創薬 / 貧血 |
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| Outline of Final Research Achievements |
In this study, we developed an in vivo drug evaluation system that uses zebrafish as a tool for drug discovery for Diamond-Blackfan anemia (DBA). (1) We performed a compound screening as a pilot study to identify candidate drugs for DBA treatment. (2) A biolabeling method was developed to detect drug metabolism in vivo. (3) We used CRISPR-Cas9 system to generate DBA models that carry rps19 gene mutation. The rps19 homozygous mutants showed severe anemia at 48 hours post fertilization. (4) The toxicity test revealed an increased lethality depending on the time period of compound treatments.
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Report
(4 results)
Research Products
(22 results)
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[Journal Article] Identification and characterization of 5alpha-cyprinol-sulfating cytosolic sulfotransferases (Sults) in the Zebrafish (Danio rerio).2017
Author(s)
Kurogi, K., Yoshihama, M., Horton, A., Schiefer, I.T., Krasowski, M.D., Hagey, L.R., Williams, F.E., Sakakibara, Y., Kenmochi, N., Suiko, M., Liu, M.-C.
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Journal Title
The Journal of Steroid Biochemistry and Molecular Biology
Volume: 174
Pages: 120-127
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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