Realization of regenerative medical system using human ES cells for congenital metabolic disorders

• Project/Area Number: 15K15398
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Pediatrics
• Research Institution: National Center for Child Health and Development
• Principal Investigator: TATUMI KUNIKO 国立研究開発法人国立成育医療研究センター, 細胞医療研究部, 共同研究員 (10534860)
• Co-Investigator(Kenkyū-buntansha): 梅澤 明弘 国立研究開発法人国立成育医療研究センター, 再生医療センター, 副所長/再生医療センター長 (70213486)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
• Keywords: システム科学 / ES細胞 / フィーダー細胞 / 未分化維持 / 再生医療 / 先天性代謝異常症 / 培養成分 / 成育医学

Outline of Final Research Achievements

In matching of stem cells and feeder cells, there is no detailed study on anatomical sites derived from feeder cells. In order to apply human ES cells to regenerative medicine, it is indispensable to construct a human ES cell culture system that eliminates foreign matter. Constructing a feeder cell assay system that keeps undifferentiated state of human ES cells plays a major role in the culture system excluding heterologous derived substances.