Generation of hair shaft-specific Cre mice

• Project/Area Number: 15K15412
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Dermatology
• Research Institution: Yamaguchi University (2016-2017); Niigata University (2015)
• Principal Investigator: SHIMOMURA Yutaka 山口大学, 大学院医学系研究科, 教授 (70397107)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 毛髪特異的 / Creマウス / Krt35 / 毛髪 / ケラチン35 / Creリコンビナーゼ

Outline of Final Research Achievements

In this project, we aimed to generate transgenic mice that specifically express Cre recombinase under the control by the promoter of a hair shaft-specific hair keratin Krt35 (Krt35-Cre). We initially made transgenic mice carrying the nucleotide sequences of Krt35-Cre. Subsequently, we backcrossed these mice with B6. 129X1-Gt(ROSA)26Sortm1 mice and successfully generated Krt35-Cre(+)/EYFP(+) mice. However, these mice did not specifically expressed EYFP in the hair shaft. Therefore we concluded that we could not achieve our objectives.