Development of techniques for analyzing somatic mutations in single neurons of psychiatric disorders
| Project/Area Number |
15K15424
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Psychiatric science
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| Research Institution | Kumamoto University |
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Principal Investigator |
Iwamoto Kazuya 熊本大学, 大学院生命科学研究部(医), 教授 (40342753)
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| Co-Investigator(Renkei-kenkyūsha) |
BUNDO Miki 熊本大学, 大学院生命科学研究部, 准教授 (00597221)
KATO Tadafumi 理化学研究所, 脳科学総合研究センター, チームリーダー (30214381)
KASAI Kiyoto 東京大学, 大学院医学系研究科, 教授 (80322056)
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| Research Collaborator |
MURATA Yui 熊本大学, 大学院生命科学研究部, 博士研究員 (10802077)
SAWAMURA Rie 熊本大学, 大学院生命科学研究部, 助教 (80509654)
KIYOTA Emi 熊本大学, 大学院生命科学研究部, 技術補佐員
UCHINUNO Emi 熊本大学, 大学院生命科学研究部, 技術補佐員
UEDA Junko 理化学研究所, 脳科学総合研究センター, 技術補佐員
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 体細胞変異 / 統合失調症 / 全ゲノム増幅 / シングルセル / 死後脳 / 神経細胞 |
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| Outline of Final Research Achievements |
In order to evaluate the physiological and pathological significance of somatic mutations occurring in brain cells, it is necessary to analyze frequency and pattern at the single cell levels in various types of brain cells. In this research project, we have established a series of analytical pipelines from pretreatment of brain samples, isolation of single neuronal nuclei, whole genome amplification, and data analysis of whole genome sequencing. These will be useful for clarifying the role of somatic mutations in the psychiatric disorders.
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Report
(4 results)
Research Products
(6 results)
