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Visualization of heavy-ion induced clustered DNA double strand break formation using super-resolution microscopy

Research Project

Project/Area Number 15K15449
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Radiation science
Research InstitutionSasaki Foundation (2016)
Gunma University (2015)

Principal Investigator

Isono Mayu  公益財団法人佐々木研究所, 附属研究所, 研究員(移行) (90713511)

Research Collaborator SHIBATA Atsushi  国立大学法人群馬大学, 医学系研究科, 助教 (30707633)
Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
KeywordsDNAクラスター損傷 / 重粒子線 / 染色体異常 / DNA修復 / ゲノム不安定性 / 超高解像度顕微鏡
Outline of Final Research Achievements

Heavy-ion radiation induces clustered DNA damage, which contains multiple type of DNA damage, e.g. DNA double strand break (DSB), single strand break and base damage. However so far, such clustered DNA damage has not been visualized in the cellular level. Here we aimed to visualize the clustered DNA damage, particularly about the formation of DSB, after heavy-ion radiation by using 3D super-resolution microscopy. To address the question whether heavy-ion irradiation generates clustered DSB formation or not, we monitored RPA foci as a DSB marker. The super-resolution analysis revealed that heavy-ion radiation induced multiple RPA foci in close proximity. Furthermore, our analysis demonstrated that the distance between two individual RPA foci within γH2AX foci was approximately 700 nm. In summary, our data suggests that these closely localized DSBs are considered to be a risk for chromosomal translocations, deletions and cell death after heavy-ion irradiation.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report

Research Products

(8 results)

All 2017 2016 2015

All Journal Article Presentation

  • [Journal Article] BRCA1 Directs the Repair Pathway to Homologous Recombination by Promoting 53BP1 Dephosphorylation2017

    • Author(s)
      Mayu Isono, Atsuko Niimi, Takahiro Oike, Yoshihiko Hagiwara, Hiro Sato, Ryota Sekine, Yukari Yoshida, Shin-Ya Isobe, Chikashi Obuse, Ryotaro Nishi, Elena Petricci, Shinichiro Nakada, Takashi Nakano, Atsushi Shibata
    • Journal Title

      Cell Rep.

      Volume: 18 Pages: 520-532

    • DOI

      10.1016/j.celrep.2016.12.042

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Carbon-ion beams effectively induce growth inhibition and apoptosis in human neural stem cells compared with glioblastoma A172 cells2015

    • Author(s)
      Isono M, Yoshida Y, Takahashi A, Oike T, Shibata A, Kubota Y, Kanai T, Ohno T, Nakano T.
    • Journal Title

      J Radiat Res

      Volume: 56 Pages: 856-861

    • DOI

      10.1093/jrr/rrv033

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] The EGFR mutation status affects the relative biological effectiveness of carbon-ion beams in non-small cell lung carcinoma cells2015

    • Author(s)
      Amornwichet N, Oike T, Shibata A, Nirodi CS, Ogiwara H, Makino H, Kimura Y, Hirota Y, Isono M, Yoshida Y, Ohno T, Kohno T, Nakano T.
    • Journal Title

      Scientific Reports

      Volume: 11 Pages: 11305-11305

    • DOI

      10.1038/srep11305

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] 炭素イオン線誘発DNA二本鎖切断の修復経路選択における線エネルギー付与の効果2016

    • Author(s)
      萩原慶彦,磯野真由,新美敦子,尾池貴洋,佐藤浩央,中野隆史,柴田淳史
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      横浜市, 神奈川県
    • Year and Date
      2016-11-30
    • Related Report
      2016 Annual Research Report
  • [Presentation] BRCA1 directs the repair pathway to homologous recombination by promoting 53BP1 dephosphorylation2016

    • Author(s)
      Mayu Isono, Atsuko Niimi, Yoshihiko Hagiwara, Ryotaro Nishi, Shinya Isobe, Chikashi Obuse, Elena Petricci, Shinichiro Nakada, Takashi Nakano and Atsushi Shibata
    • Organizer
      The 10th 3R Symposium
    • Place of Presentation
      Matsue, Shimane, Japan
    • Year and Date
      2016-11-13
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The effect of Linear Energy Transfer in DNA double strand break repair pathway choice in G2 cells2016

    • Author(s)
      Yoshihiko Hagiwara, Atsuko Niimi, Mayu Isono, Takahiro Oike, Hiro Sato, Takashi Nakano and Atsushi Shibata
    • Organizer
      The 10th 3R Symposium
    • Place of Presentation
      Matsue, Shimane, Japan
    • Year and Date
      2016-11-13
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] BRCA1によるDSB end resectionの時空間的制御2015

    • Author(s)
      磯野真由
    • Organizer
      第38回 日本分子生物学会・第88回日本生化学会 合同大会
    • Place of Presentation
      神戸ポートピアホテル 本館 B1F 偕楽1(神戸市)
    • Year and Date
      2015-12-03
    • Related Report
      2015 Research-status Report
  • [Presentation] The complexity of damage at the DNA break ends induces rapid DNA double strand break resection2015

    • Author(s)
      磯野真由
    • Organizer
      ICRR2015
    • Place of Presentation
      国立京都国際会議場(京都市)
    • Year and Date
      2015-05-25
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research

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Published: 2015-04-16   Modified: 2018-03-22  

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