| Budget Amount *help |
¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
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| Outline of Final Research Achievements |
Membrane-fluidity sensitive boron liposomes (MFSBLs) had a mean diameter of 59.6 nm and a zeta potential of -11.3 mV. High encapsulation efficiency value from 55% to 89% of B-10 in MFSBLs were obtained. MFSBLs had high stability (95-99%) in the retention of B-10 during storage at 4°C for 30 d. All borocap-tate-loaded formulations had low cytotoxic effects in human fibroblast cells. MFSBLs were efficiently fused to melanoma cells, but were inefficiently fused to human fibroblast cells. Thus, it is essential to elevate the B-10 concentration in melanoma cells, while maintain low levels of B-10 in normal fibroblast cells. The tumor/normal ratio (T/N ratio) was 3.0. MFSBLs showed higher suppression of growth of melanoma cells than BSH solution. This result suggested novel MFSBLs composed of DMPC is useful for B-10 carrier on BNCT for melanoma.
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