| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
Fluoroacetate (FACE) has been known to be incorporated into astrocyte and acts as a metabolic toxin by blocking the tricarboxylic acid cycle. The aim of this study was to determine whether 18F-labelled FACE ([18F]FACE) is useful for a novel diagnostic imaging marker in acute cerebral ischemia. Cerebral ischemia model rats were made by occluding the right middle cerebral artery (MCA) for 60 min followed by subsequent reperfusion. [18F]FACE-PET scan was conducted under isoflurane anesthesia. [18F]FACE was highly accumulated in the ischemic region during MCA occlusion with low cerebral flow, in which no [18F]FACE metabolite was observed. Histological analyses revealed that [18F]FACE accumulation was not associated with appearance of reactive astrocyte and breakdown of blood brain barrier. These finding indicate that ischemia causes changes in astrocyte energy metabolism and also alter FACE transportation, in other words [18F]FACE can be a promising PET probe in acute cerebral ischemia.
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