Establishing a new cryopreservation method for pig pancreatic islet sheet by vitrification and an evaluation method by a high-throughput sequencing.

• Project/Area Number: 15K15480
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General surgery
• Research Institution: Meiji University
• Principal Investigator: Masaki Nagaya 明治大学, 研究・知財戦略機構, 特任教授 (90329300)
• Co-Investigator(Kenkyū-buntansha): 渡辺 將人 明治大学, 研究・知財戦略機構, 特任講師 (00321688) ; 長嶋 比呂志 明治大学, 農学部, 専任教授 (50318664) ; 梅山 一大 明治大学, 研究・知財戦略機構, 特任准教授 (70342699) ; 松成 ひとみ 明治大学, 研究・知財戦略機構, 特任講師 (70639517) ; 新井 良和 明治大学, 研究・知財戦略機構, 研究推進員(ポスト・ドクター) (90614769)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 膵ランゲルハンス島 / 膵島移植 / 膵頭シート / 凍結保存 / ガラス化凍結 / 膵島シート

Outline of Final Research Achievements

Islet transplantation is considered as an option for the treatment of type 1 diabetes. However, transplanted islets face several challenges, including Blood type and Human leukocyte antigen mismatches. In addition, islet isolation is still complicated and technically difficult in some cases. Therefore, the cases of mismatches and islets with a low yield at isolation are not transplanted and distributed for basic research. To allow the unlimited collection of islets, islet cryopreservation methods need to be optimized. In this regards, the pancreatic islet sheet may provide a promising option for cryopreserving islets. A library of cryopreserved islets allows for selection based on HLA tissue type and creates more flexibility regarding the total amount of transplantable mass. The present study aimed at establishing a new cryopreservation method for pig pancreatic islet sheet by vitrification and an evaluation method by a high-throughput sequencing

Research Products

• [Journal Article] Diabetic phenotype of transgenic pigs introduced by dominant-negative mutant hepatocyte nuclear factor 1α. (2017)
  • Author(s): Umeyama K, Nakajima M, Yokoo T, Nagaya M, Nagashima H.
  • Journal Title: J Diabetes Complications. 2017 Volume: 16 Pages: 30529-3
  • Peer Reviewed / Open Access
• [Journal Article] An Effective New Cryopreservation Procedure for Pancreatic Islets Using Hollow Fiber Vitrification. (2016)
  • Author(s): Nagaya M, Matsunari H, Kanai T, Maehara M, Nakano K, Umeki I, Katsumata Y, Kasai Y, Sakai R, Kobayashi M, Honda M, Abe N, Watanabe M, Umeyama K, Nagashima H.
  • Journal Title: Horm Metab Res. Volume: 48 Issue: 08 Pages: 540-9
  • DOI: 10.1055/s-0042-102628
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A transgenic-cloned pig model expressing non-fluorescent modified Plum. (2016)
  • Author(s): Nagaya M, Watanabe M, Kobayashi M, Nakano K, Arai Y, Asano Y, Takeishi T, Umeki I, Fukuda T, Yashima S, Takayanagi S, Watanabe N, Onodera M, Matsunari H, Umeyama K, Nagashima H.
  • Journal Title: J Reprod Dev. Volume: 62 Pages: 511-520
  • NAID: 130005431253
  • Peer Reviewed / Open Access
• [Presentation] ブタにおけるゲノム編集技術を用いた遺伝子ノックアウト (2016)
  • Author(s): 1.渡邊將人、松成ひとみ、中野和明、梅山一大、高柳就子、長屋昌樹、宮川周士、花園 豊、中内啓光、長嶋比呂志
  • Organizer: 日本ゲノム編集学会 第1回大会
  • Place of Presentation: 広島
  • Year and Date: 2016-09-06