| Project/Area Number |
15K15484
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Digestive surgery
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Noda Natsumi 東京大学, 大学院理学系研究科(理学部), 特任研究員 (30624358)
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| Co-Investigator(Renkei-kenkyūsha) |
OZAWA Takeaki 東京大学, 大学院理学系研究科, 教授 (40302806)
TAKAORI Kyoichi 京都大学, 健康長寿社会の総合医療開発ユニット, 准教授 (10329485)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 遺伝子発現制御 / 光遺伝学 / Phytochrome B / 光受容体 / 癌 / ルシフェラーゼ / がん / 光制御 / 遺伝子発現 / 膵癌 |
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| Outline of Final Research Achievements |
Pancreatic cancer is difficult to detect at an early stage and is a disease with a very poor prognosis. Therefore, it is desired to develop a new pancreatic cancer therapy. Spatiotemporal control of transcription using optogenetic tools may be a powerful technology for cancer therapy. We developed an optogenetic tool based on Arabidopsis thaliana phytochrome (Phy) B and its binding partner, phytochrome-interacting factor (PIF) 6. We generated a truncated PhyB, which allowed for reversible association with PIF6 by red/far-red light illumination. The red light illumination only for 5 min induced PhyB translocation from cytoplasm into the nucleus by the association with PIF6, resulting in transcriptional activation based on Gal4 DNA-binding domain and the upstream activating sequence of Gal system. The nucleocytoplasmic shuttling vector using PhyB and PIF6 may be applicable for transcriptional regulation in biological processes.
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