iPSC derived three dimensional cardiac tissue having circulatory system

• Project/Area Number: 15K15506
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Cardiovascular surgery
• Research Institution: Osaka University
• Principal Investigator: Miyagawa Shigeru 大阪大学, 医学系研究科, 特任教授(常勤) (70544237)
• Co-Investigator(Kenkyū-buntansha): 福嶌 五月 国立研究開発法人国立循環器病研究センター, 病院, 医長 (80596867) ; 秦 広樹 大阪大学, 医学系研究科, 助教 (80638198)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: iPS細胞 / in vivo / iPS由来三次元心筋組織 / 重症心不全 / 心機能回復 / ３次元心筋組織 / 毛細血管網 / 細胞外基質リモデリング / 三次元組織化

Outline of Final Research Achievements

Despite a remarkable progress in the treatment for severe heart failure such as heart transplantation and left ventricular assist device, heart failure is still a leading cause of mortality. Recently, it has become possible to make organ tissue outside the body by remarkable innovation of cell engineering technology and a spread usage of human iPSC.In this study, we made a human iPSC derived 3D myocardial tissue chip in vitro, and we could find a good drug responsiveness of the chip compared with the conventional monolayer cardiomyocytes. It suggests the utility as a drug screening system.
Next, we transplanted the human iPSC-derived three-dimensional myocardial tissue on the heart to heart failure models, and we could find the recovery of cardiac function. The implanted cardiac tissue after one month also remained in vivo, and the maturation was recognized compared with the tissue in vitro.

Research Products

• [Presentation] ヒトiPS細胞由来3次元心筋組織を用いた薬剤安全性スクリーニングシステムの検討 (2016)
  • Author(s): 武田真季、宮川繁、福嶌五月、齋藤充弘、増田茂夫、李鍾國、今西悠基子、伊東絵望子、原田明希摩、小田紀子、天野雄斗、松崎典弥、明石満、澤芳樹
  • Organizer: 第15回日本再生医療学会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2016-03-17
• [Presentation] 再生医学を用いた重症心不全に対する挑戦 (2015)
  • Author(s): 宮川繁
  • Organizer: 第53回日本人工臓器学会
  • Place of Presentation: 東京ドームホテル
  • Year and Date: 2015-11-21
  • Invited
• [Presentation] Development of In Vitro Drug-Induced Cardiotoxicity Assay by Using Three-Dimensional Cardiac Tissues Derived from Human Induced Pluripotent Stem Cells (2015)
  • Author(s): Takeda M, Miyagawa S, Fukushima S, Ito E, Matsuura R, Harada A, Amano Y, Matsusaki M, Akashi M,
  • Organizer: American Heart Association Scientific Sessions 2015
  • Place of Presentation: Orlando, USA
  • Year and Date: 2015-11-07
  • Int'l Joint Research
• [Presentation] iPS細胞を用いた新しい心不全治療法の開発 (2015)
  • Author(s): 宮川繁
  • Organizer: 第19回日本心不全学会
  • Place of Presentation: 大阪
  • Year and Date: 2015-10-19
  • Invited
• [Presentation] Development of Functional Tissue-engineered Artificial Cardiac Construct generated by Human Induced Pluripotent Stem Cells (2015)
  • Author(s): Iseok H, Miyagawa S, Fukushima S, Saito A, Ito E, Sawa Y
  • Organizer: TERMIS
  • Place of Presentation: Boston, USA
  • Year and Date: 2015-09-08
  • Int'l Joint Research