Novel in vivo optical imaging method monitoring oxidative stress

• Project/Area Number: 15K15527
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Neurosurgery
• Research Institution: Okayama University
• Principal Investigator: Abe Koji 岡山大学, 医歯薬学総合研究科, 教授 (20212540)
• Co-Investigator(Kenkyū-buntansha): 山下 徹 岡山大学, 医歯薬学総合研究科, 講師 (60644408)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: in vivo イメージング / 脳梗塞 / Keap1 / Nrf2 / 酸化ストレスセンサー / 中大脳動脈閉塞モデル / NRF2 / 酸化ストレス / 一過性中大脳動脈閉塞

Outline of Final Research Achievements

We investigated the time-dependent change of in vivo optical imaging of oxidative stress after stroke with OKD mice. The in vivo signals showed a peak at 1 d after tMCAO that was slightly correlated to infarct volume. The strong ex vivo signals, which were detected in the peri-ischemic area, corresponded to endogenous Nrf2 expression.
Our result of synchronization between in vivo imaging and endogenous Nrf2 expression might provide a benefit to verification of drugs targeting the Nrf2-mediated defense pathway and the therapeutic time window in acute phase of ischemic stroke.

Research Products

• [Journal Article] Time-dependent change of in vivo optical imaging of oxidative stress in a mouse stroke model. (2017)
  • Author(s): Nakano Y, Yamashita T, Li Q, Sato K, Ohta Y, Morihara R, Hishikawa N, Abe K.
  • Journal Title: J Neurosci Res. Volume: 印刷中 Issue: 10 Pages: 2030-2039
  • DOI: 10.1002/jnr.24047
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Neuroprotective Effects of a Novel Antioxidant Mixture Twendee X in Mouse Stroke Model. (2017)
  • Author(s): Kusaki M, Ohta Y, Inufusa H, Yamashita T, Morihara R, Nakano Y, Liu X, Shang J, Tian F, Fukui Y, Sato K, Takemoto M, Hishikawa N, Abe K.
  • Journal Title: J Stroke Cerebrovasc Dis. Volume: 印刷中 Issue: 6 Pages: 1191-1196
  • DOI: 10.1016/j.jstrokecerebrovasdis.2017.01.003
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Reduction of intracerebral hemorrhage by rivaroxaban after tPA thrombolysis is associated with downregulation of PAR-1 and PAR-2. (2016)
  • Author(s): Morihara R, Yamashita T, Kono S, Shang J, Nakano Y, Sato K, Hishikawa N, Ohta Y, Heitmeier S, Perzborn E, Abe K.
  • Journal Title: J Neurosci Res. Volume: 印刷中 Issue: 9 Pages: 1818-1828
  • DOI: 10.1002/jnr.24013
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Dynamic mislocalizations of nuclear pore complex proteins after focal cerebral ischemia in rat. (2016)
  • Author(s): Li Q, Ohta Y, Yamashita T, Shang J, Deguchi K, Feng T, Sato K, Hishikawa N, Nakano Y, Abe K.
  • Journal Title: J Neurosci Res. Volume: 印刷中 Issue: 9 Pages: 1745-1759
  • DOI: 10.1002/jnr.24005
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Temporal Profiles of Stress Protein Inductions after Focal Transient Ischemia in Mice Brain. (2016)
  • Author(s): Li Q, Nakano Y, Shang J, Ohta Y, Sato K, Takemoto M, Hishikawa N, Yamashita T, Abe K.
  • Journal Title: J Stroke Cerebrovasc Dis. Volume: Oct;25(10): Issue: 10 Pages: 2344-2351
  • DOI: 10.1016/j.jstrokecerebrovasdis.2016.05.031
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Recent Progress in Therapeutic Strategies for Ischemic Stroke. (2016)
  • Author(s): Yamashita T, Abe K.
  • Journal Title: Cell Transplant. Volume: 25 Issue: 5 Pages: 893-8
  • DOI: 10.3727/096368916x690548
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Protective effect of telmisartan on neurovascular unit and inflammasome in stroke-resistant spontaneously hypertensive rats. (2015)
  • Author(s): Liu W, Yamashita T, Kurata T, Kono S, Hishikawa N, Deguchi K, Zhai Y, Abe K.
  • Journal Title: Neurol Res. Volume: 37 Issue: 6 Pages: 491-501
  • DOI: 10.1179/1743132815y.0000000002
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] High Incidence of Dementia Conversion than Stroke Recurrence in Poststroke Patients of Late Elder Society. (2015)
  • Author(s): Nakano Y, Deguchi K, Yamashita T, Morihara R, Matsuzono K, Kawahara Y, Sato K, Kono S, Hishikawa N, Ohta Y, Higashi Y, Takao Y, Abe K.
  • Journal Title: J Stroke Cerebrovasc Dis. Volume: 24 Issue: 7 Pages: 1621-8
  • DOI: 10.1016/j.jstrokecerebrovasdis.2015.03.037
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Telmisartan promotes potential glucose homeostasis in stroke-resistant spontaneously hypertensive rats via peroxisome proliferator-activated receptor γ activation. (2015)
  • Author(s): Omote Y, Deguchi K, Kurata T, Yamashita T, Sato K, Hishikawa N, Abe K.
  • Journal Title: Curr Neurovasc Res. Volume: 12 Pages: 91-7
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Neurovascular protection by telmisartan via reducing neuroinflammation in stroke-resistant spontaneously hypertensive rat brain after ischemic stroke. (2015)
  • Author(s): Kono S, Kurata T, Sato K, Omote Y, Hishikawa N, Yamashita T, Deguchi K, Abe K.
  • Journal Title: J Stroke Cerebrovasc Dis. Volume: 24 Issue: 3 Pages: 537-47
  • DOI: 10.1016/j.jstrokecerebrovasdis.2014.09.037
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] ラット一過性脳虚血モデルにおけるTDP43とFUS/TLSの発現亢進による神経細胞保護効果について (2016)
  • Author(s): 中野由美子、孫淼、山下徹、森原隆太、太田康之、阿部康二
  • Organizer: 第59回日本脳循環代謝学会学術集会
  • Place of Presentation: 徳島
  • Year and Date: 2016-11-11
• [Presentation] ivaroxaban and Apixaban Reduce Hemorrhagic Complication by Protection of Neurovascular Unit after Thrombolysis in Ischemic Stroke of Rat (2016)
  • Author(s): Morihara R, Kono S, Yamashita T, and Abe K
  • Organizer: The 9th International Symposium on Neuroprotection Neurorepair 2016
  • Place of Presentation: Leipzig, Germany
  • Year and Date: 2016-04-19
  • Int'l Joint Research
• [Presentation] Novel cell transplantation therapy for stroke using induced neural stem cells (2016)
  • Author(s): Yamashita T, Morihara R and Abe K
  • Organizer: The 9th International Symposium on Neuroprotection Neurorepair 2016
  • Place of Presentation: Leipzig, Germany
  • Year and Date: 2016-04-19
  • Int'l Joint Research
• [Presentation] Thrombolysis with Tissue Plasminogen Activator 3-4.5h after Acute Ischemic Stroke in 5 Hospital Groups in Japan (2015)
  • Author(s): Morihara R, Kono S, Nakano Y, Yamashita T, Manabe Y, Takao Y, Kashihara K,
  • Organizer: Asia Pacific Stroke Conference 2015
  • Place of Presentation: Kuala Lumpur, Malaysia
  • Year and Date: 2015-10-02
  • Int'l Joint Research
• [Presentation] High incidence of dementia conversion than stroke recurrence in post-stroke patients of late elder society (2015)
  • Author(s): Nakano Y, Deguchi K, Yamashita T, Morihara R, Sato K, Hishikawa N, Ohta Y, Higashi Y, Takao Y, and Abe K
  • Organizer: The 3rd Asian Clinical Conference (ACC3)
  • Place of Presentation: Tokyo, JAPAN
  • Year and Date: 2015-09-03
  • Int'l Joint Research
• [Presentation] Strong improvement of ApoE/LDL-R signals by telmisartan in post-stroke SHR-SR (2015)
  • Author(s): Yamashita T, Zhai Y, Hishikawa N, Ohta Y, Abe K
  • Organizer: The 3rd Asian Clinical Conference (ACC3)
  • Place of Presentation: Tokyo, JAPAN
  • Year and Date: 2015-09-03
  • Int'l Joint Research
• [Presentation] In vivo optical imaging in post-stroke mice model treated with bone marrow stromal cells or edaravone (2015)
  • Author(s): Yamashita T, Tian F, Deguchi K, Ohta Y, Hishikawa N, Abe K
  • Organizer: The 9th International Conference on Complex Medical Engineering 2015 (CME2015)
  • Place of Presentation: Tokyo, JAPAN
  • Year and Date: 2015-06-18
  • Int'l Joint Research