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Elucidation of temporo-spatial heterogeneity of glioma by Liquid Biopsy

Research Project

Project/Area Number 15K15529
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Neurosurgery
Research InstitutionKyushu University

Principal Investigator

MIZOGUCHI Masahiro  九州大学, 医学研究院, 共同研究員 (50380621)

Co-Investigator(Kenkyū-buntansha) 佐山 徹郎  九州大学, 医学研究院, 講師 (30346788)
吉本 幸司  九州大学, 大学病院, 講師 (70444784)
天野 敏之  九州大学, 医学研究院, 共同研究員 (70448413)
橋口 公章  九州大学, 大学病院, 講師 (80448422)
Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsglioma / liquid biopsy / cell free DNA / microRNA / digital PCR / グリオーマ / 脳腫瘍
Outline of Final Research Achievements

We started Liquid Biopsy by circulating cell free DNA (cfDNA) and microRNA (miRNA) analysis in body fluids for glioma cases. Firstly, global expression analysis of blood miRNA revealed that expression of many miRNA is elevated in glioma cases, and some specific miRNAs could be analyzed also in cerebrospinal fluid. Secondly, cfDNA analysis was undertaken. First, mutation analysis of IDH1 was carried out using the TaqMan method, but due to the small amount of cfDNA, it was difficult to accurately evaluate the mutation, and most of the cases were not analyzable. Next, we introduced digital PCR, and it was clarified that mutation analysis of IDH1 gene is possible by analysis of cfDNA in cerebrospinal fluid.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (16 results)

All 2017 2016 2015

All Journal Article (7 results) (of which Peer Reviewed: 7 results,  Acknowledgement Compliant: 6 results,  Open Access: 4 results) Presentation (9 results) (of which Invited: 1 results)

  • [Journal Article] Prevalence and clinicopathological features of H3.3 G34-mutant high-grade gliomas: a retrospective study of 411 consecutive glioma cases in a single institution.2017

    • Author(s)
      Yoshimoto K, Hatae R, Sangatsuda Y, Suzuki SO, Hata N, Akagi Y, Kuga D, Hideki M, Yamashita K, Togao O, Hiwatashi A, Iwaki T, Mizoguchi M, Iihara K.
    • Journal Title

      Brain Tumor Pathol.

      Volume: 印刷中 Issue: 3 Pages: 103-112

    • DOI

      10.1007/s10014-017-0287-7

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] A comprehensive analysis identifies BRAF hotspot mutations associated with gliomas with peculiar epithelial morphology.2016

    • Author(s)
      Hatae R, Hata N, Suzuki SO, Yoshimoto K, Kuga D, Murata H, Akagi Y, Sangatsuda Y, Iwaki T, Mizoguchi M, Iihara K.
    • Journal Title

      Neuropathology

      Volume: 28 Issue: 3 Pages: 191-199

    • DOI

      10.1111/neup.12347

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Deferred radiotherapy and upfront procarbazine-ACNU-vincristine administration for 1p19q codeleted oligodendroglial tumors are associated with favorable outcome without compromising patient performance, regardless of WHO grade.2016

    • Author(s)
      Hata N, Yoshimoto K, Hatae R, Kuga D, Akagi Y, Suzuki SO, Iwaki T, Shono T, Mizoguchi M, Iihara K.
    • Journal Title

      Onco Targets Ther.

      Volume: 9 Pages: 7123-7131

    • DOI

      10.2147/ott.s115911

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Insular primary glioblastomas with IDH mutations: Clinical and biological specificities.2016

    • Author(s)
      Hata N, Hatae R, Yoshimoto K, Murata H, Kuga D, Akagi Y, Sangatsuda Y, Suzuki SO, Iwaki T, Mizoguchi M, Iihara K.
    • Journal Title

      Neuropathology

      Volume: 24 Issue: 3 Pages: 200-207

    • DOI

      10.1111/neup.12362

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Add-on bevacizumab can prevent early clinical deterioration and prolong survival in newly diagnosed partially resected glioblastoma patients with a poor performance status.2016

    • Author(s)
      Hata N, Yoshimoto K, Hatae R, Kuga D, Akagi Y, Sangatsuda Y, Suzuki SO, Shono T, Mizoguchi M, Iihara K.
    • Journal Title

      Onco Targets Ther.

      Volume: 10 Pages: 429-437

    • DOI

      10.2147/ott.s125587

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Detection of proneural/mesenchymal marker expression in glioblastoma: temporospatial dynamics and association with chromatin-modifying gene expression2015

    • Author(s)
      Hideki Murata・Koji Yoshimoto・Ryusuke Hatae・Yojiro Akagi・Masahiro Mizoguchi・Nobuhiro Hata・Daisuke Kuga・Akira Nakamizo・Toshiyuki Amano・Tetsuro Sayama・Koji Iihara
    • Journal Title

      Journal of Neuro-oncology

      Volume: 125 Issue: 1 Pages: 33-41

    • DOI

      10.1007/s11060-015-1886-y

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] 分子病理学に基づくグリオーマ分類2015

    • Author(s)
      溝口昌弘
    • Journal Title

      脳神経外科ジャーナル

      Volume: 24 Pages: 366-377

    • NAID

      130005083639

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Presentation] グリオーマ400例における1p/19q共欠失の解析2016

    • Author(s)
      吉本幸司、溝口昌弘
    • Organizer
      第34回脳腫瘍学会
    • Place of Presentation
      甲府富士屋ホテル
    • Year and Date
      2016-12-04
    • Related Report
      2016 Annual Research Report
  • [Presentation] 初発神経膠芽腫に対するBevacizumab併用療法の治療成績2016

    • Author(s)
      秦暢宏、溝口昌弘
    • Organizer
      第34回脳腫瘍学会
    • Place of Presentation
      甲府富士屋ホテル
    • Year and Date
      2016-12-04
    • Related Report
      2016 Annual Research Report
  • [Presentation] IDHmutant, 1p19qcodeleted oligodendrogliomaの長期予後に関する検討2016

    • Author(s)
      空閑太亮、溝口昌弘
    • Organizer
      第34回脳腫瘍学会
    • Place of Presentation
      甲府富士屋ホテル
    • Year and Date
      2016-12-04
    • Related Report
      2016 Annual Research Report
  • [Presentation] 1p19q co-deletionを有するolidodendrogliomaに対するPAV療法:分子診断による治療層別化の長期成績2016

    • Author(s)
      秦暢宏、溝口昌弘
    • Organizer
      第34回脳腫瘍病理学会
    • Place of Presentation
      東京カンファレンスセンター有明
    • Year and Date
      2016-05-27
    • Related Report
      2016 Annual Research Report
  • [Presentation] pGBMにおけるIDH/TERT promoter/ch10 LOHの解析2015

    • Author(s)
      波多江龍亮、秦暢宏、赤木洋二郎、村田秀樹、空閑太亮、吉本幸司、溝口昌弘、飯原弘二
    • Organizer
      第33回日本脳腫瘍学会学術集会
    • Place of Presentation
      京都府京都市
    • Year and Date
      2015-12-06
    • Related Report
      2015 Research-status Report
  • [Presentation] ベバシズマブ時代における悪性神経膠腫の診断と治療2015

    • Author(s)
      溝口昌弘
    • Organizer
      日本脳神経外科学会第74回学術総会
    • Place of Presentation
      北海道札幌市
    • Year and Date
      2015-10-14
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] IDH/TERT変異解析による、グリオーマの分子病理学的層別化:255症例の検討2015

    • Author(s)
      波多江龍亮、秦暢宏、赤木洋二郎、村田秀樹、空閑太亮、吉本幸司、溝口昌弘、飯原弘二
    • Organizer
      日本脳神経外科学会第74回学術総会
    • Place of Presentation
      北海道札幌市
    • Year and Date
      2015-10-14
    • Related Report
      2015 Research-status Report
  • [Presentation] グリオーマ診断における分子病理学の役割2015

    • Author(s)
      溝口昌弘、吉本幸司、村田秀樹、波多江龍亮、赤木洋次郎、天野敏之、飯原弘二
    • Organizer
      第33回日本脳腫瘍病理学会
    • Place of Presentation
      香川県高松市
    • Year and Date
      2015-05-29
    • Related Report
      2015 Research-status Report
  • [Presentation] 神経膠芽腫症例におけるBRAF遺伝子点突然変異解析2015

    • Author(s)
      波多江龍亮、秦暢宏、鈴木諭、赤木洋二郎、村田秀樹、天野敏之、吉本幸司、溝口昌弘、飯原弘二
    • Organizer
      第33回日本脳腫瘍病理学会
    • Place of Presentation
      香川県高松市
    • Year and Date
      2015-05-29
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2018-03-22  

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