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Analysis for activated astrocytes -with nano surgery method-

Research Project

Project/Area Number 15K15532
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Neurosurgery
Research InstitutionWaseda University

Principal Investigator

IKESHIMA Hiroko  早稲田大学, 理工学術院, その他(招聘研究員) (60265783)

Project Period (FY) 2015-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywordsアストロサイト / 二光子レーザー顕微鏡 / レーザーアブレーション(ナノサージェリー法) / 足突起 / 脳血管 / 血液脳関門 / アウトロサイト / アブレーション / in vivoイメージング / レーザーアブレーション / 二光子顕微鏡 / マウス / レーザー / トランスジェニックマウス
Outline of Final Research Achievements

Astrocyte plays functional roles such as provision of nutrients from blood vessels to neurons, supplying neurotransmitters and supporting blood brain barrier (BBB) integrity. Astrocytic endfeet are known to support the integrity of BBB through maintenance of the tight junction between endothelial cells of blood vessels but evidence of its direct contribution is still unclear owing to technical limitations. In this study, astrocytic endfoot covering blood vessels were removed by the laser ablation (nano surgery method) with two photon laser microscopy in in vivo mouse brain, and re-covering of blood vessels with astrocytic endfeet was observed. BBB kept their integrity without astrocytic endfoot covers: leakage of blood vessel marker dyes from blood vessels was not observed during blood vessels were stripped, while dyes leaked when the vascular walls were ablated. These results suggest that astrocytic endfoot covering blood vessels do not contribute to the immediate BBB barrier.

Academic Significance and Societal Importance of the Research Achievements

本研究では,レーザーアブレーションを初めてアストロサイトに適用した.これまでの遺伝子改変マウスを用いた解析とは異なり,生きたマウス脳内のたった一つのアストロサイト足突起の挙動を追跡することが可能である.今後さらに,アストロサイトの機能を明らかにする上で有用な手法であると考える.
BBBの存在により,脳神経疾患に対する薬の投与に対してはバリアーとなることが治療を困難にしている.本研究では脳血管へのアブレーションによって,BBBを一時的に破綻させることを可能にした.将来的には本法をヒト用に改良して,BBBが破綻しているうちに脳内へ薬を投与して脳神経疾患の治療に応用することを目指している.

Report

(5 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (35 results)

All 2019 2018 2017 2016 2015

All Journal Article (7 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 7 results,  Open Access: 7 results,  Acknowledgement Compliant: 3 results) Presentation (28 results) (of which Int'l Joint Research: 16 results,  Invited: 6 results)

  • [Journal Article] Metabolic Plasticity of Astrocytes and Aging of the Brain2019

    • Author(s)
      Morita Mitsuhiro、Ikeshima-Kataoka Hiroko、Kreft Marko、Vardjan Nina、Zorec Robert、Noda Mami
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 20 Issue: 4 Pages: 941-941

    • DOI

      10.3390/ijms20040941

    • NAID

      120006599242

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Astrocytic endfeet re-cover blood vessels after removal by laser ablation2019

    • Author(s)
      Hideaki Kubotera#, Hiroko Ikeshima-Kataoka#*, Yoshiki Hatashita, Anna Letizia Allegra Mascaro, Francesco Saverio Pavone, Takafumi Inoue* (#These authors are equally contributed, *Corresponding authors)
    • Journal Title

      Scientific Reports

      Volume: 9 Issue: 1

    • DOI

      10.1038/s41598-018-37419-4

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Osteopontin is indispensable for activation of astrocytes in injured mouse brain and primary culture2018

    • Author(s)
      Hiroko Ikeshima-Kataoka*, Yutaka Matsui, Toshimitsu Uede (*Corresponding author)
    • Journal Title

      Neurological Research

      Volume: 40 Issue: 12 Pages: 1071-1079

    • DOI

      10.1080/01616412.2018.1517995

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 2-carba cyclic phosphatidic acid suppresses inflammation via regulation of microglial polarization in the stab-wounded mouse cerebral cortex2018

    • Author(s)
      Kei HASHIMOTO, Mari NAKASHIMA, Ayana HAMANO, Mari GOTOH, Hiroko IKESHIMA-KATAOKA, Kimiko MURAKAMI-MUROFUSHI, Yasunori MIYAMOTO
    • Journal Title

      Scientific Reports

      Volume: 8 Issue: 1 Pages: 9715-9715

    • DOI

      10.1038/s41598-018-27990-1

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Correlation between astrocyte activity and recovery from blood-brain barrier breakdown caused by brain injury2016

    • Author(s)
      Hiroko Ikeshima-Kataoka* and Masato Yasui:
    • Journal Title

      Neuroreport

      Volume: 27 Issue: 12 Pages: 894-900

    • DOI

      10.1097/wnr.0000000000000619

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Neuroimmunological Implications of AQP4 in Astrocytes2016

    • Author(s)
      Hiroko Ikeshima-Kataoka
    • Journal Title

      Int. J. Mol. Sci.

      Volume: 17 Issue: 8 Pages: 1-16

    • DOI

      10.3390/ijms17081306

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Aquaporin 4-Dependent Expression of Glial Fibrillary Acidic Protein and Tenascin-C in Activated Astrocytes in Stab Wound Mouse Brain and in Primary Culture2015

    • Author(s)
      Hiroko Ikeshima-Kataoka*, Yoichiro Abe, Masato Yasui (* corresponding author)
    • Journal Title

      Journal of Neuroscience Research

      Volume: 93 Issue: 1 Pages: 121-129

    • DOI

      10.1002/jnr.23467

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Neuroimmunological function of osteopontin in activation of astrocytes in stab wound mouse brain and LPS stimulated primary culture2019

    • Author(s)
      Hiroko Ikeshima-Kataoka, Motoko Furukawa, Sayaka Inui, Yutaka Matsui, Toshimitsu Uede, Masato Yasui
    • Organizer
      13th Gottingen Meeting of the German Neuroscience Society
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Reactive Astrocyte has a Neuroimmunological Function in injured Mouse Brain2018

    • Author(s)
      Hiroko IKESHIMA-KATAOKA (invited speaker at Plenary Lecture), Motoko FURUKAWA, Sayaka INUI, Manae IMAMURA, Yoko HONJYO, Masato YASUI
    • Organizer
      World Summit on Neurology & Neuroscience
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Activated Astrocytes has a Neuroimmunological Function in Stab Wounded Mouse Brain2018

    • Author(s)
      Hiroko IKESHIMA-KATAOKA (invited symposiast, chairperson and received “BEST PRESENTATION AWARD”), Motoko FURUKAWA, Sayaka INUI, Manae IMAMURA, Yoko HONJYO, Masato YASUI
    • Organizer
      ICTBISN 2018: 20th International Conference on Traumatic Brain Injury and Sports Neurology
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Neuroimmunological function of osteopontin in activation of astrocytes in stab wound mouse brain and LPS stimulated primary culture2018

    • Author(s)
      Hiroko Ikeshima-Kataoka, Motoko Furukawa, Sayaka Inui, Yutaka Matsui, Toshimitsu Uede, Masato Yasui
    • Organizer
      Cold Spring Harbor-Asia Conference, Novel Insights into Glia Function & Dysfunction
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 2-carba cyclic phosphatidic acid contributes to the repair of stab-wounded cerebral cortex via regulation of microglial and astrocyte cells2018

    • Author(s)
      Mari Nakashima, Kei Hashimoto, Ayana Hamano, Hiroko Ikeshima-Kataoka, Mari Gotoh, Kimiko Murakami-Murofushi, Yasunori Miyamoto
    • Organizer
      The American Society for Cell Biology
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Characteristics of astrocytic endfeet in mouse brain revealed by laser ablation2018

    • Author(s)
      Hideaki KUBOTERA, Hiroko IKESHIMA-KATAOKA, Yoshiki HATASHITA, Anna Letizia ALLEGRA MASCARO, Francesco PAVONE, Takafumi INOUE
    • Organizer
      Society for Neuroscience
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Re-covering blood vessels by astrocytic endfeet after laser ablation2018

    • Author(s)
      Hideaki KUBOTERA, Hiroko IKESHIMA-KATAOKA, Anna Letizia ALLEGRA MASCARO, Francesco PAVONE, Takafumi INOUE
    • Organizer
      18th World Congress of Basic and Clinical Pharmacology
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Astrocyte Reactivation is Concerned in BBB Recovery from Breakdown2018

    • Author(s)
      Hiroko IKESHIMA-KATAOKA (received “Best Poster Presentation Award”), Motoko FURUKAWA, Sayaka INUI, Yoko HONJYO, Manae IMAMURA and Masato YASUI
    • Organizer
      2nd International Conference on Neurology and Brain Disorders
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The effect of carba-derivative of cyclic phosphatidic acid on microglial and astrocyte cells during the repair of a stab-wounded mouse cerebral cortex2018

    • Author(s)
      Mari Nakashima, Kei Hashimoto, Ayana Hamano, Hiroko Ikeshima-Kataoka, Mari Gotoh, Kimiko Murakami-Murofushi, Yasunori Miyamoto
    • Organizer
      第61回日本神経化学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Analysis of the relationship between astrocytic endfoot and blood vessel2018

    • Author(s)
      Hideaki KUBOTERA, Hiroko IKESHIMA-KATAOKA, Anna Letizia ALLEGRA MASCARO, Francesco PAVONE, Takafumi INOUE
    • Organizer
      第41回日本神経科学大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Vitronectin regulates the fibrinolytic system in the stab-wounded mouse cerebral cortex2018

    • Author(s)
      Kei Hashimoto, Natsumi Ikeda, Mari Nakashima, Hiroko Ikeshima-Kataoka, Yasunori Miyamoto
    • Organizer
      第50回日本結合組織学会学術大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Re-covering blood vessels by astrocytic endfeet after laser ablation2018

    • Author(s)
      Hideaki KUBOTERA, Hiroko IKESHIMA-KATAOKA, Anna Letizia ALLEGRA MASCARO, Francesco PAVONE, Takafumi INOUE
    • Organizer
      18th World Congress of Basic and Clinical Pharmacology in Kyoto
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Analysis of the relationship between astrocytic endfoot and blood vessel2018

    • Author(s)
      Hideaki KUBOTERA, Hiroko IKESHIMA-KATAOKA, Anna Letizia ALLEGRA MASCARO, Francesco PAVONE, Takafumi INOUE
    • Organizer
      第41回日本神経科学学会
    • Related Report
      2017 Research-status Report
  • [Presentation] Laser ablation newly identified functional role of astrocytic endfeet in mouse brain2018

    • Author(s)
      Hideaki KUBOTERA, Hiroko IKESHIMA-KATAOKA, Yoshiki HATASHITA, Anna Letizia ALLEGRA MASCARO, Francesco PAVONE, Takafumi INOUE
    • Organizer
      Society for Neuroscience
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Activated astrocytes concern in recovery from BBB breakdown in mouse brain injury2017

    • Author(s)
      Hiroko Ikeshima-Kataoka, Motoko Furukawa, Sayaka Inui, Manae Imamura, Masato Yasui
    • Organizer
      第90回日本薬理学会大会
    • Place of Presentation
      長崎
    • Year and Date
      2017-03-15
    • Related Report
      2016 Research-status Report
  • [Presentation] Functional analysis of astrocyte endfoot for the blood brain barrier integrity in mouse brain2017

    • Author(s)
      Hideaki KUBOTERA, Hiroko IKESHIMA-KATAOKA, Anna Letizia ALLEGRA MASCARO, Francesco PAVONE, Takafumi INOUE
    • Organizer
      第40回日本神経科学学会
    • Related Report
      2017 Research-status Report
  • [Presentation] Astrocyte Reactivation has a Neuroimmunological Role in Injured Mouse Brain2016

    • Author(s)
      Hiroko IKESHIMA-KATAOKA (invited symposiast and chairperson), Motoko FURUKAWA, Sayaka INUI, Manae IMAMURA, Yoko HONJYO, Masato YASUI
    • Organizer
      BIT’s 7th World Gene Convention 2016
    • Place of Presentation
      Shanghai, China
    • Year and Date
      2016-11-03
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Functional role of activated astrocytes for blood-brain barrier breakdown after the brain injury2016

    • Author(s)
      Hiroko IKESHIMA-KATAOKA, Motoko Furukawa, Sayaka INUI, Manae IMAMURA, Masato YASUI
    • Organizer
      第38回日本生物学的精神医学会 ・第59回日本神経化学会合同大会
    • Place of Presentation
      福岡
    • Year and Date
      2016-09-08
    • Related Report
      2016 Research-status Report
  • [Presentation] Functional analysis of activated astrocytes induced by brain injury for blood-brain barrier2016

    • Author(s)
      Hiroko IKESHIMA-KATAOKA, Motoko Furukawa, Sayaka INUI, Manae IMAMURA, Masato YASUI
    • Organizer
      第39回日本神経科学大会
    • Place of Presentation
      横浜
    • Year and Date
      2016-07-20
    • Related Report
      2016 Research-status Report
  • [Presentation] Analysis of regulatory mechanism for upregulation of TN-C and GFAP expression in activated astrocytes in injured mouse brain and in primary culture2016

    • Author(s)
      Hiroko IKESHIMA-KATAOKA, Motoko FURUKAWA, Sayaka INUI, Manae IMAMURA and Masato YASUI
    • Organizer
      10th FENS Forum of Neuroscience
    • Place of Presentation
      Copenhagen, Denmark
    • Year and Date
      2016-07-02
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Functional Role of Reactive Astrocytes in Injured Mouse Brain2016

    • Author(s)
      Hiroko IKESHIMA-KATAOKA (invited symposiast), Motoko FURUKAWA, Sayaka INUI, Manae IMAMURA, Yoko HONJYO, Masato YASUI
    • Organizer
      9th Annual World Protein & Peptide Conference
    • Place of Presentation
      Dalian, China
    • Year and Date
      2016-04-25
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Functional role of reactive astrocytes in injured mouse brain2016

    • Author(s)
      Hiroko IKESHIMA-KATAOKA ,Motoko FURUKAWA, Sayaka INUI, Manae IMAMURA, Yoko HONJYO, Masato YASUI
    • Organizer
      9th Annual World Protein & Peptide Conference
    • Place of Presentation
      Dalian International Conference Center, Dalian, China
    • Year and Date
      2016-04-25
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Functional role of activated astrocytes in blood-brain barrier breakdown caused by brain injury2016

    • Author(s)
      Hiroko IKESHIMA-KATAOKA, Motoko Furukawa, Sayaka INUI, Manae IMAMURA, Masato YASUI
    • Organizer
      第89回日本薬理学会年会
    • Place of Presentation
      パシフィコ横浜 会議センター,神奈川県
    • Year and Date
      2016-03-09
    • Related Report
      2015 Research-status Report
  • [Presentation] 損傷脳組織における活性化アストロサイトの機能解析―アクアポリン4に着目して-2016

    • Author(s)
      池島(片岡)宏子,古川元子,乾さやか,今村愛枝,安井正人
    • Organizer
      第129回日本薬理学会近畿部会
    • Place of Presentation
      広島
    • Related Report
      2016 Research-status Report
  • [Presentation] Upregulation of TN-C and GFAP in reactive astrocytes in injured brain and in primary culture is dependent on aquaporin-4 expression2015

    • Author(s)
      Hiroko IKESHIMA-KATAOKA, Motoko Furukawa, Sayaka INUI, Manae IMAMURA, Masato YASUI
    • Organizer
      第58回日本神経化学会大会
    • Place of Presentation
      大宮ソニックシティー,埼玉県
    • Year and Date
      2015-09-11
    • Related Report
      2015 Research-status Report
  • [Presentation] Functional analysis of activated astrocytes in injured mouse brain2015

    • Author(s)
      Hiroko IKESHIMA-KATAOKA
    • Organizer
      3D Exchange Symposium
    • Place of Presentation
      Biopolis (Aspiration Theatrette, Matrix Bldg), Singapore
    • Year and Date
      2015-09-07
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Functional analysis of molecules around injury site in the CNS2015

    • Author(s)
      Hiroko IKESHIMA-KATAOKA, Sayaka INUI, Manae IMAMURA, Masato YASUI
    • Organizer
      第38回日本神経科学大会
    • Place of Presentation
      神戸国際会議場,神戸国際展示場,兵庫県
    • Year and Date
      2015-07-28
    • Related Report
      2015 Research-status Report
  • [Presentation] Functional analysis of TN-C and GFAP induced upregulation in the reactive astrocytes in the injured brain and in primary culture2015

    • Author(s)
      Hiroko IKESHIMA-KATAOKA, Sayaka INUI, Manae IMAMURA, Masato YASUI
    • Organizer
      12th European Meeting on Glial Cells in Health and Disease
    • Place of Presentation
      The Euskalduna Conference Centre, Bilbao, Spain
    • Year and Date
      2015-07-15
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research

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Published: 2015-04-16   Modified: 2020-03-30  

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