Gait analyses of osteoarthritis model mice for joint pain assessment

• Project/Area Number: 15K15535
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Orthopaedic surgery
• Research Institution: The University of Tokyo
• Principal Investigator: SAITO Taku 東京大学, 医学部附属病院, 講師 (30456107)
• Co-Investigator(Kenkyū-buntansha): 柳原 大 東京大学, 大学院総合文化研究科, 准教授 (90252725)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 整形外科学 / 変形性関節症 / 動作解析

Outline of Final Research Achievements

Joint pain is the most common symptom of OA, however, molecular mechanisms of pain in OA joint have been generally unrevealed. Here, we performed the motion analysis of surgically-induced OA model mice to examine how cartilage degeneration of the knee joint alters gait and joint motions. Locomotion recording and Motion analyses were performed at six months after osteoarthritis surgery. Among all parameters, the degree of knee flexion in the stepping-in phase was significantly changed in the OA group.

Research Products

• [Journal Article] Different regulation of limb development by p63 transcript variants (2017)
  • Author(s): Kawata M, Taniguchi Y, Mori D, Yano F, Ohba S, Chung UI, Shimogori T, Mills AA, Tanaka S, Saito T
  • Journal Title: PLoS One Volume: 12 Issue: 3 Pages: e0174122-e0174122
  • DOI: 10.1371/journal.pone.0174122
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Targeting gene expression to specific cells of kidney tubules in vivo, using adenoviral promoter fragments. (2017)
  • Author(s): Sumiyo Watanabe , Toru Ogasawara, Yoshifuru Tamura, Taku Saito, Toshiyuki Ikeda, Nobuchika Suzuki, Tatsuo Shimosawa, Shigeru Shibata, Ung-il Chung, Masaomi Nangaku, Shunya Uchida
  • Journal Title: PLoS One Volume: 12 Issue: 3 Pages: 1-14
  • DOI: 10.1371/journal.pone.0168638
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Regulation of Chondrocyte Survival in Mouse Articular Cartilage by p63. (2017)
  • Author(s): Taniguchi Y, Kawata M, Chang SH, Mori D, Okada K, Kobayashi H, Sugita S, Hosaka Y, Inui H, Taketomi S, Yano F, Ikeda T, Akiyama H, Mills AA, Chung UI, Tanaka S, Kawaguchi H, and Saito T*
  • Journal Title: Arthritis Rheumatol. Volume: 69 Issue: 3 Pages: 598-609
  • DOI: 10.1002/art.39976
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Biphasic regulation of chondrocytes by Rela through induction of anti-apoptotic and catabolic target genes. (2016)
  • Author(s): Kobayashi H, Chang S, Mori D, Itoh S, Hirata M, Hosaka Y, Taniguchi Y, Okada K, Mori Y, Yano F, Chung U, Akiyama H, Kawaguchi H, Tanaka S, Saito T.
  • Journal Title: Nat Commun Volume: 7 Issue: 1 Pages: 13336-13336
  • DOI: 10.1038/ncomms13336
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Messenger RNA delivery of a cartilage-anabolic transcription factor as a disease-modifying strategy for osteoarthritis treatment. (2016)
  • Author(s): 1.Aini H, Itaka K, Fujisawa A, Uchida H, Uchida S, Fukushima S, Kataoka K, Saito T, Chung UI, Ohba S.
  • Journal Title: Sci Rep. Volume: 6 Issue: 1 Pages: 18743-18743
  • DOI: 10.1038/srep18743
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Comparison of mouse and human ankles and establishment of mouse ankle osteoarthritis models by surgically-induced instability (2016)
  • Author(s): Chang SH, Yasui T, Taketomi S, Matsumoto T, Kim-Kaneyama JR, Omiya T, Hosaka Y, Inui H, Omata Y, Yamagami R, Mori D, Yano F, Chung U, Tanaka S, Saito T.
  • Journal Title: Osteoarthritis Cartilage Volume: 24 Issue: 4 Pages: 688-697
  • DOI: 10.1016/j.joca.2015.11.008
  • Peer Reviewed
• [Journal Article] <b>Hyaline cartilage formation and tumorigenesis of implanted tissues derived from human induced pluripotent stem </b><b>cells </b> (2015)
  • Author(s): Saito T, Yano F, Mori D, Kawata M, Hoshi K, Takato T, Masaki H, Otsu M, Eto K, Nakauchi H, Chung UI, Tanaka S
  • Journal Title: Biomedical Research Volume: 36 Issue: 3 Pages: 179-186
  • DOI: 10.2220/biomedres.36.179
  • NAID: 130005083858
  • ISSN: 0388-6107, 1880-313X
  • Peer Reviewed / Open Access
• [Presentation] 過剰な力学的ストレス負荷による関節軟骨変性の分子メカニズム (2016)
  • Author(s): 齋藤琢
  • Organizer: 第39回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2016-12-02
  • Invited
• [Presentation] 変性性関節症の制御メカニズム (2016)
  • Author(s): 齋藤琢
  • Organizer: Orthopedic Research Club
  • Place of Presentation: 木更津
  • Year and Date: 2016-11-19
  • Invited
• [Presentation] 過剰な力学的ストレスによる関節軟骨異化のメカニズム (2016)
  • Author(s): 齋藤琢
  • Organizer: 第10回骨軟骨フロンティア
  • Place of Presentation: 東京
  • Year and Date: 2016-11-05
  • Invited
• [Presentation] Regulation of Articular Cartilage Homeostasis and Osteoarthritis Development by the NF-kappaB signaling. (2016)
  • Author(s): Taku Saito
  • Organizer: 2016 Cold Spring Harbor Asia Conference on Bone & Cartilage
  • Place of Presentation: Suzhou, China
  • Year and Date: 2016-10-26
  • Int'l Joint Research / Invited
• [Presentation] 過剰な力学的ストレスによる関節軟骨異化のメカニズム (2016)
  • Author(s): 齋藤琢
  • Organizer: 第34回日本骨代謝学会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2016-07-21
  • Invited
• [Presentation] Perspective of disease-modifying osteoarthritis drugs (2016)
  • Author(s): Taku Saito
  • Organizer: 第3回アジア太平洋骨代謝学会議
  • Place of Presentation: 大阪
  • Year and Date: 2016-07-20
  • Int'l Joint Research / Invited
• [Presentation] Gait Analyses of Surgically Induced osteoarthritis model mice by motion capture system. (2015)
  • Author(s): Makii Y, Saito T, et al.
  • Organizer: Annual meeting of Osteoarthritis Research Society International.
  • Place of Presentation: Washington State Convention Center (Seattle, WA)
  • Year and Date: 2015-04-30
  • Int'l Joint Research
• [Remarks] 東京大学医学部整形外科
  • URL: http://www.u-tokyo-ortho.jp/