| Project/Area Number |
15K15542
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
Inose Hiroyuki 東京医科歯科大学, 大学院医歯学総合研究科, 助教 (30615711)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 骨代謝 / ノンコーディングRNA / 骨芽細胞 / 長鎖ノンコーディングRNA |
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| Outline of Final Research Achievements |
Recently, long non-coding RNAs (lncRNAs) have been shown to regulate various developmental and metabolic events. Osteoblast differentiation is an indispensable process in proper skeletal development and acquisition of bone mass, however, the physiological role of lncRNAs in osteoblast differentiation have not yet to be fully understood. Here, we found that CRNDE is an important regulator of this process through comprehensive analysis of lncRNAs expression during osteoblast differentiation by RNAseq. CRNDE was expressed in osteoblasts, and its expression increased over the course of osteoblast differentiation. Overexpression of CRNDE in osteoblasts promoted their differentiation, and on the opposite side, deletion of CRNDE expression inhibited osteoblast differentiation. Finally, we established CRNDE knockout mice using CRISPR/Cas9, and will continue to investigate its phenotype in further research. Our data shows that lncRNA is an important regulator of osteoblast differentiation.
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