Establishment of in vivo imaging and quantification of articular cartilage by using a cartilage-specific probe.
| Project/Area Number |
15K15551
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Orthopaedic surgery
|
|---|
| Research Institution | Okayama University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
西田 圭一郎 岡山大学, 医歯薬学総合研究科, 准教授 (80284058)
加来田 博貴 岡山大学, 医歯薬学総合研究科, 准教授 (80362961)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
HIROHATA Satoshi 岡山大学, 大学院保健学研究科, 教授 (90332791)
OHTSUKI Takakashi 岡山大学, 大学院保健学研究科, 客員研究員 (10534802)
|
|---|
| Research Collaborator |
ASZDI Attila ミュンヘン大学, 外科学再生医療部門, 教授
|
|---|
| Project Period (FY) |
2015-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
|---|
| Keywords | 軟骨変性 / プロテオグリカン / X線 / nano CT / 関節軟骨 / Ｘ線 / 組織再生 / プローブ |
|---|
| Outline of Final Research Achievements |
A new X-ray imaging probe 2Ke2-TIB has been created in addition to Ke4-TIB. These probes were examined for in vivo imaging of articular cartilage by CT using rat osteoarthritic model. A new patent regarding Ke4-TIB has been established on March 24th, 2017. Since we speculate the increasing use of mouse genetic model for drug discovery research, we created a new mouse genetic model by crossing floxed "A" gene mice, encoding a proteoglycan gene abundant in the articular cartilage, with Rosa26-creERT mice. The mice exhibited a dwarfism and articular cartilage degeneration after starting injection of tamoxifen at one week of age. These developments of new imaging probes and a mouse model will contribute to the future research on osteoarthritis drug discovery. A collaboration with a group in the University of Munich has started for nano Xray CT.
|
Report
(3 results)
Research Products
(5 results)
