| Project/Area Number |
15K15565
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Anesthesiology
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| Research Institution | Niigata University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
馬場 洋 新潟大学, 医歯学系, 教授 (00262436)
渡部 達範 新潟大学, 医歯学総合病院, 助教 (30748330)
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| Co-Investigator(Renkei-kenkyūsha) |
SHIBUKI Katsuei 新潟大学, 脳研究所, 教授 (40146163)
TAKEBAYASHI Hirohide 新潟大学, 医歯学系, 教授 (60353439)
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| Research Collaborator |
SASAKI Mika
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | カルシウムイメージング / 遺伝子導入 / 疼痛メカニズム / 脊髄後角 / 一次知覚神経 |
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| Outline of Final Research Achievements |
In this study, we aimed to introduce calcium-sensitive fluorescent protein into the spinal dorsal horn and/or dorsal root ganglia (DRG) using adeno-associated virus vector (AAV) to measure the neural activity in the spinal dorsal horn two-dimensionally. We found that the AAV vector inoculated intrathecal cavity seemed to express TurboRFP, which is a marker protein, both in the spinal cord dorsal horn and DRG 1 to 2 weeks after administration. However, due to limitations of immunohistological techniques, we could not be confirmed that the transgene-derived protein was definitely expressed in spinal dorsal horn and DRG. Therefore, it did not reach the result of the physiological experiment that was planned ahead.
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