| Project/Area Number |
15K15572
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Anesthesiology
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| Research Institution | Oita University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
朴 天鎬 北里大学, 獣医学部, 准教授 (50383550)
野口 賀津子 南九州大学, 健康栄養科学部, 助手(移行) (00760943)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 慢性疼痛 / 遺伝子治療 / 狂犬病ウイルス / G蛋白質 / 知覚神経指向性 / ウイルスベクター / レンチウイルスベクター / N型糖鎖 |
|---|
| Outline of Final Research Achievements |
In this work, we assessed a lentiviral vector pseudotyped with the G protein of the rabies virus strain 1088, which prefers to infect sensory neurons, as a vector for gene therapy for chronic pain. We succeeded in production of infectious lentiviral particles with the G protein and found that an N-glycan addition on the G protein slightly enhanced the virus production. However, the virus production by the modified 1088 G protein was still significantly lower than that by the G protein of vesicular stomatitis virus. In order to assess using animal experiments, we consider that some improvements are needed to increase the production of the pseudotyped virus.
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