| Project/Area Number |
15K15578
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Urology
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| Research Institution | Hokkaido University |
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Principal Investigator |
Mino Kazuhiro 北海道大学, 医学研究科, 客員研究員 (80750380)
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| Co-Investigator(Kenkyū-buntansha) |
嶋村 剛 北海道大学, 大学病院, 准教授 (00333617)
深井 原 北海道大学, 医学研究科, 特任助教 (60374344)
木村 太一 北海道大学, 医学研究科, 特任助教 (90435959)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 移植・再生医療 / 外科 / 発現制御 / 臓器灌流 / 虚血再灌流 / 臓器保存 |
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| Outline of Final Research Achievements |
Human renal tubular cell line (HK-2) or 14-3-3ζ overexpressed HK-2 (ζHK-2) were subjected to cold preservation in UW solution for 72 hours. Cellular death, ATP content, and MTT catabolism were evaluated.Despite possessing many potentially protective functions, 14-3-3ζ worsen the cellular injury under hypothermic oxygenated condition. 14-3-3ζ accelerates mitochondrial functions together with iron-dependent oxidative damage.The upregulation of 14-3-3ζ could be a method to maintain mitochondrial function under hypothermic oxygenated condition, shown in HMP of renal grafts, when appropriate antioxidant treatment is applied.
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