Hydrogen sulfide has a role as an endogenous relaxation factor in the bladder and prostate of male rats

• Project/Area Number: 15K15583
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Urology
• Research Institution: Kochi University
• Principal Investigator: Saito Motoaki 高知大学, 教育研究部医療学系基礎医学部門, 教授 (60273893)
• Co-Investigator(Kenkyū-buntansha): 清水 孝洋 高知大学, 教育研究部医療学系基礎医学部門, 准教授 (00363276) ; 東 洋一郎 高知大学, 教育研究部医療学系基礎医学部門, 助教 (80380062) ; 清水 翔吾 高知大学, 教育研究部医療学系基礎医学部門, 助教 (90721853)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 硫化水素 / 膀胱 / 前立腺 / 弛緩 / 硫化水素合成酵素

Outline of Final Research Achievements

We found that: (1) MPST and CAT were detected in the bladder and prostate and CBS was only detected in the prostate. On the other hand, CSE and DAO were not detected in both tissues. Immunoreactvities of these enzymes were mainly detected in the urothelium and smooth muscle layer of the bladder, and in the prostate glandular epithelium. (2) H2S was detected in the bladder and prostate tissues. (3) NaHS dose-dependently induced relaxation of pre-contracted BL-D, BL-T, PR-D and PR-V strips. There were no significant differences of the EC50 values or maximal relaxation rate against the pre-contractions among any of these tissues. Intravesically instilled GYY4137 significantly prolonged intercontraction intervals compared to the vehicle-treated group, and showed, although non-significant, tendency to reduce maximal voiding pressurecompared to the vehicle-treated group.

Research Products

• [Presentation] Hydrogen sulfide can be an endogenous relaxation factor in the rat bladder and prostate. (2017)
  • Author(s): Shimizu T, Zou S, Shimizu S, Higashi Y, Nakamura K, Aratake T, Yamamoto M, Hamada T, Nagao Y, Saito M.
  • Organizer: ICS2017 (International Continence Society 47th Annual Meeting)
  • Int'l Joint Research
• [Presentation] 硫化水素は膀胱および前立腺における内因性の弛緩因子である (2017)
  • Author(s): 清水孝洋、Zou Suo、清水翔吾、東洋一郎、尾野秀彬、中村久美子、新武享朗、山本雅樹、濱田朋弥、長尾佳樹、齊藤源顕
  • Organizer: 第９回排尿障害モデル動物研究会
• [Presentation] Endogenous hydrogen sulfide can function as a relaxation factor in the rat bladder and prostate (2017)
  • Author(s): Zou S, Shimizu T, Shimizu S, Higashi Y, Ono H, Nakamura K, Aratake T, Yamamoto M, Hamada T, Nagao Y, Saito M
  • Organizer: 第７０回日本薬理学会西南部会
• [Presentation] Wistar雄性ラットにおける膀胱および前立腺に対する硫化水素の弛緩作用と硫化水素合成酵素の発現 (2016)
  • Author(s): 中村久美子
  • Organizer: 第89回日本薬理学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県・横浜市）
  • Year and Date: 2016-03-09
• [Presentation] 硫化水素によるラット膀胱および前立腺に対する弛緩作用と硫化水素産生酵素の発現分布 (2015)
  • Author(s): 中村久美子
  • Organizer: 第68回日本薬理学会西南部会
  • Place of Presentation: 海峡メッセ下関（山口県・下関市）
  • Year and Date: 2015-11-21
• [Presentation] Endogenous hydrogen sulfide can function as a potent relaxing factor in the rat bladder and prostate (2015)
  • Author(s): 清水孝洋
  • Organizer: The 10th Pan-Pacific Continence Society Meeting
  • Place of Presentation: 札幌コンベンションセンター（北海道・札幌）
  • Year and Date: 2015-09-07
  • Int'l Joint Research
• [Presentation] Hydrogen sulfide is a potent relaxing factor in the rat bladder and prostate (2015)
  • Author(s): 清水孝洋
  • Organizer: American Urological Association, 110th Annual Meeting
  • Place of Presentation: New Orleans
  • Year and Date: 2015-05-15
  • Int'l Joint Research