| Project/Area Number |
15K15588
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Urology
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| Research Institution | International University of Health and Welfare |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
佐藤 陽子 東亜大学, 公私立大学の部局等, 教授 (50398963)
吉池 美紀 聖マリアンナ医科大学, 医学部, 研究技術員 (60398964)
大西 公平 慶應義塾大学, 理工学部(矢上), 教授 (80137984)
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| Co-Investigator(Renkei-kenkyūsha) |
MORIKAWA Yasuhide 慶應義塾大学, 医学部, 講師 (90124958)
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| Research Collaborator |
NAKAZAWA Ryuto 聖マリアンナ医科大学, 医学部, 講師
NOZAWA Shiari 聖マリアンナ医科大学, 医学部, 研究員
OGAWA Kenji 慶應義塾大学, 大学院・理工学研究科, 大学院生
TOMIZUKA Daisuke 慶應義塾大学, 大学院・理工学研究科, 大学院生
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 非閉塞性無精子症 / 顕微鏡下精巣内精子回収術 / 精巣硬度 / 造精機能障害モデル停留精巣 / Johnsen score / haptics / 精子回収率 / scarling / 精細管硬度 / 精細管基底膜 / 停留精巣造精機能障害モデル / 精細管基底膜肥厚 / haptics技術 |
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| Outline of Final Research Achievements |
Microdissection testicular sperm extraction (MD-TESE) is the first choice treatment for patients with non-obstructive azoospermia. The identification of seminiferous tubules in which spermatozoa present is dependent on subjective observation under a microscope. As an objective index to raise the sperm retrieval rate in MD-TESE, it was devised to evaluate the hardness of seminiferous tubule by precise mechanical measurement. As a first step, we tried hardness measurement on the testes by pushing testes with a device using a micro force sensor and measuring the reaction force. Experimental cryptorchidism hamster model was prepared to measure the hardness and spermatogenic potential was evaluated by Johnsen score (JS). Result： the cryptorchidism group showed a significantly lower hardness and JS than the untreated group, and a strong positive correlation was found between hardness and JS.
It was shown that the hardness is useful as a new index of the spermatogenic evaluation.
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