| Project/Area Number |
15K15592
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
岡江 寛明 東北大学, 医学系研究科, 助教 (10582695)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 生殖医学 / エピジェネティクス |
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| Outline of Final Research Achievements |
Placental is common as the interface between mother and fetus to obtain nutrients essential for embryonic growth and gene expression is regulated by epigenetic mechanism. DNA methylation is a kind of epigenetic factors and globally reprogrammed after fertilization. Genomic imprinting is also leads to parent-of-origin specific mono-allelic expression of certain genes by DNA methylation (DMR). In this study, we performed genome-wide allelic DNA methylation analyses of human germ cells, blastocyst and purified trophoblast cells from placentas. We found that incomplete demethylation in btastocyst. RNA sequencing-based allelic expression analyses revealed that some of the placenta-specific maternal DMRs were associated with imprinted gene expression. These findings highlight the incomplete erasure of germline DNA methylation in the human placenta, which is important for understanding normal placental development and the pathogenesis of developmental disorders with imprinting effects.
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