DNA methylation analyses in the human placenta cells

• Project/Area Number: 15K15592
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Obstetrics and gynecology
• Research Institution: Tohoku University
• Principal Investigator: Arima Takahiro 東北大学, 医学系研究科, 教授 (80253532)
• Co-Investigator(Kenkyū-buntansha): 岡江 寛明 東北大学, 医学系研究科, 助教 (10582695)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 生殖医学 / エピジェネティクス

Outline of Final Research Achievements

Placental is common as the interface between mother and fetus to obtain nutrients essential for embryonic growth and gene expression is regulated by epigenetic mechanism. DNA methylation is a kind of epigenetic factors and globally reprogrammed after fertilization. Genomic imprinting is also leads to parent-of-origin specific mono-allelic expression of certain genes by DNA methylation (DMR). In this study, we performed genome-wide allelic DNA methylation analyses of human germ cells, blastocyst and purified trophoblast cells from placentas. We found that incomplete demethylation in btastocyst. RNA sequencing-based allelic expression analyses revealed that some of the placenta-specific maternal DMRs were associated with imprinted gene expression. These findings highlight the incomplete erasure of germline DNA methylation in the human placenta, which is important for understanding normal placental development and the pathogenesis of developmental disorders with imprinting effects.

Research Products

• [Journal Article] Polyunsaturated Fatty Acid Levels in Maternal Erythrocytes of Japanese Women during Pregnancy and after Childbirth. (2017)
  • Author(s): Kawabata T, Kagawa Y, Kimura F, Miyazawa T, Saito S, Arima T, Nakai K, Yaegashi N
  • Journal Title: Nutrients Volume: 9 Issue: 3 Pages: 1-10
  • DOI: 10.3390/nu9030245
  • Peer Reviewed / Open Access
• [Journal Article] Factors associated with aberrant imprint methylation and oligozoospermia (2017)
  • Author(s): Kobayashi N, Miyauchi N, Tatsuta N, Kitamura A, Okae H, Hiura H, Sato A, Utsunomiya T, Yaegashi N, Nakai K, Arima T
  • Journal Title: Scientific Reports Volume: 7 Issue: 1 Pages: 42336-42336
  • DOI: 10.1038/srep42336
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Genome-Scale Assessment of Age-Related DNA Methylation Changes in Mouse Spermatozoa. (2016)
  • Author(s): Kobayashi N, Okae H, Hiura H, Chiba H, Shirakata Y, Hara K, Tanemura K, Arima T.
  • Journal Title: PLoS One Volume: 11 Issue: 11 Pages: e0167127-e0167127
  • DOI: 10.1371/journal.pone.0167127
  • Peer Reviewed / Open Access
• [Journal Article] Incidence of Domestic Violence Against Pregnant Females After the Great East Japan Earthquake in Miyagi Prefecture: The Japan Environment and Children's Study (2016)
  • Author(s): Sakurai K, Nishigori H, Nishigori T, Mizuno S, Obara T, Iwama N, Watanabe Z, Ishikuro M, Tatsuta N, Nishijima I, Sugawara J, Fujiwara I, Arima T, Kuriyama S, Metoki H, Takahashi F, Nakai K, Yaegashi N; Japan Environment & Children’s Study Group
  • Journal Title: Disaster Med Public Health Prep Volume: 27 Issue: 2 Pages: 1-11
  • DOI: 10.1017/dmp.2016.109
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Association between social capital and the prevalence of gestational diabetes mellitus: An interim report of the Japan Environment and Children's Study (2016)
  • Author(s): Mizuno S, Nishigori H, Sugiyama T, Takahashi F, Iwama N, Watanabe Z, Sakurai K, Ishikuro M, Obara T, Tatsuta N, Nishijima I, Fujiwara I, Arima T, Kuriyama S, Metoki H, Nakai K, Inadera H, Yaegashi N; Japan Environment & Children’s Study Group
  • Journal Title: Diabetes Res Clin Pract Volume: 120 Pages: 132-41
  • DOI: 10.1016/j.diabres.2016.07.020
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Allele-specific methylome and transcriptome analysis reveals widespread imprinting in the human placenta (2016)
  • Author(s): Hamada, H., Okae, H., Toh, H., Chiba, H., Hiura, H., Shirane, K., Sato, T., Suyama, M., Yaegashi, N., Sasaki, H. & Arima
  • Journal Title: The American Journal of Human Genetics Volume: 99 Issue: 5 Pages: 1045-1058
  • DOI: 10.1016/j.ajhg.2016.08.021
  • Peer Reviewed / Open Access
• [Journal Article] DNA methylation dynamics during early human development (2016)
  • Author(s): Okae H, Arima T
  • Journal Title: Journal of Mammalian Ova Research Volume: 33 Pages: 2-2
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Prevalence and Determinants of Inadequate Use of Folic Acid Supplementation in Japanese Pregnant Women: The Japan Environment and Children's Study (JECS) (2016)
  • Author(s): Obara T, Nishigori H, Nishigori T, Metoki H, Ishikuro M, Tatsuta N, Mizuno S, Sakurai K, Nishijima I, Murai Y, Fujiwara I, Arima T, Nakai K, Mano N, Yaegashi N, Kuriyama S, JECS group
  • Journal Title: J Matern Fetal Neonatal Med Volume: 30 Issue: 5 Pages: 588-593
  • DOI: 10.1080/14767058.2016.1179273
• [Journal Article] DNA methylation errors in imprinting disorders and male infertility. (2015)
  • Author(s): Kitamura A, Hiura H, Miyauchi N, Okae H, Chiba H, Arima T.
  • Journal Title: Congenit Anom (Kyoto) Volume: 55 Pages: 133-144
• [Journal Article] Heterogeneity of the Fatty Acid Composition of Japanese Placentae for Determining the Perinatal Fatty Acid Status: a Methodological Study. (2015)
  • Author(s): Yamazaki I, Kimura F, Nakagawa K, Nakai K, Arima T, Kawabata T, Kagawa Y, Saitoh S, Mizuno S, Yaegashi N, Miyazawa T.
  • Journal Title: J Oleo Sci. Volume: 64 Pages: 905-14
  • NAID: 130005090062
  • Peer Reviewed / Open Access
• [Presentation] ヒト凍結融解胚移植胎盤におけるmicroRNAの網羅的解析 (2016)
  • Author(s): 樋浦仁、服部裕充、岡江寛明、千葉初音、宮内尚子、北村茜、菊地裕幸、吉田秋仁、有馬隆博
  • Organizer: 第39回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-11-30
• [Presentation] 繁殖期におけるマウス精子DNAメチル化様式の加齢性変化 (2016)
  • Author(s): 小林記緒、岡江寛明、樋浦仁、千葉初音、白形芳樹、原健士郎、種村健太郎、有馬隆博
  • Organizer: 第39回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-11-30
• [Presentation] 分化多能性を持つヒト胎盤栄養膜幹細胞の樹立 (2016)
  • Author(s): 岡江寛明、有馬隆博
  • Organizer: 第39回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-11-30
  • Invited
• [Presentation] ウシ卵胞内卵子の発生能を制御する卵丘細胞シグナルネットワーク (2016)
  • Author(s): 杉村智史、岡江寛明、有馬隆博
  • Organizer: 第109回日本繁殖生物学会大会
  • Place of Presentation: 麻布大学（神奈川県相模原市）
  • Year and Date: 2016-09-11
• [Presentation] Incomplete reprogramming of germline DNA methylation in the human placenta (2016)
  • Author(s): Arima T, Okae H
  • Organizer: IHEC Science Days & Annual Meeting 2016
  • Place of Presentation: Crowne Plaza Brussels-LePalace(Belgian, Brussels)
  • Year and Date: 2016-09-06
  • Int'l Joint Research
• [Presentation] Transcriptome profiling of purified human trophoblast cells (2016)
  • Author(s): Hamada H, Sato T, Okae H, Yaegashi N, and Arima T.
  • Organizer: 63th Annual Scientific Meeting
  • Place of Presentation: Montreal, QC, Canada
  • Year and Date: 2016-03-16
  • Int'l Joint Research
• [Presentation] ヒト胚発生におけるエピジェネティクスの変調 (2015)
  • Author(s): 有馬隆博
  • Organizer: 第33回受精着床学会
  • Place of Presentation: TFTホール（東京都江東区）
  • Year and Date: 2015-11-26
  • Invited
• [Presentation] ヒト胎盤におけるインプリント型X染色体不活性化 (2015)
  • Author(s): 濱田裕貴、岡江寛明、八重樫伸生、有馬隆博
  • Organizer: 第23回日本胎盤学会学術集会
  • Place of Presentation: JA共済ビル（東京都千代田区）
  • Year and Date: 2015-11-05
• [Presentation] 子どもの健康と環境に関する全国調査から見えてくるもの (2015)
  • Author(s): 有馬隆博
  • Organizer: 第25回日本新生児看護学会
  • Place of Presentation: マリオス（岩手県盛岡市）
  • Year and Date: 2015-10-24
  • Invited
• [Presentation] DNA methylation dynamics and regulation of genomic imprinting during human development (2015)
  • Author(s): Okae H, Arima T
  • Organizer: EMBO Workshop
  • Place of Presentation: Montreal, QC, Canada
  • Year and Date: 2015-05-06
  • Int'l Joint Research
• [Book] 先端医療シリーズ48「臨床医のための最新産科婦人科」 (2017)
  • Author(s): 濱田裕貴、高橋聡太、服部裕充、有馬隆博
  • Publisher: 先端医療技術研究所
• [Book] 生殖補助医療(ART) -胚培養の理論と実際- (2017)
  • Author(s): 有馬隆博、樋浦仁、岡江寛明、佐藤晶子
  • Total Pages: 352
  • Publisher: 近代出版
• [Book] 産科と婦人科 Vol.84 No.1 (2016)
  • Author(s): 有馬隆博
  • Publisher: 診断と治療社
• [Book] Pharma Medica (2016)
  • Author(s): 有馬隆博、宮内尚子、北村茜、樋浦仁、岡江寛明、千葉初音
  • Total Pages: 144
  • Publisher: メディカルレビュー社
• [Book] 小児科臨床 (2015)
  • Author(s): 樋浦仁、宮内尚子、有馬隆博
  • Total Pages: 8
  • Publisher: 日本小児医事出版社
• [Book] 生命誌ジャーナル (2015)
  • Author(s): 有馬隆博
  • Total Pages: 1
  • Publisher: JT生命誌研究館